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PXD014001

PXD014001 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVascular rays mediate radial assimilate transport in tuberous roots of Manihot esculenta
DescriptionCassava (Manihot esculenta) is one of the most important staple food crops worldwide. Its starchy tuberous roots supply over 800 million people with carbohydrates. Yet, surprisingly little is known about the processes involved in filling of those vital storage organs. A better understanding of cassava carbohydrate allocation and starch storage is key to improve storage root yield. In this work, we studied cassava morphology and phloem sap flow from source to sink using transgenic pAtSUC2::GFP plants, the phloem tracers esculin and 5(6)-carboxyfluorescein diacetate (CFDA), as well as several staining techniques. We show that cassava performs apoplasmic phloem loading in source leaves and symplasmic unloading into phloem parenchyma cells of tuberous roots. We demonstrate that vascular rays play an important role in radial transport from the phloem to xylem parenchyma cells in tuberous roots. Furthermore, enzymatic and proteomic measurements of storage root tissues confirmed high abundance and activity of enzymes involved in the sucrose synthase-mediated pathway and indicated that starch is stored most efficiently in the outer xylem layers of tuberous roots. Our findings represent a first basis for biotechnological approaches aimed at improved phloem loading and enhanced carbohydrate allocation and storage in order to increase tuberous root yield of cassava.
HostingRepositoryPRIDE
AnnounceDate2019-07-08
AnnouncementXMLSubmission_2019-07-08_08:22:24.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD014001
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristian Lamm
SpeciesList scientific name: Manihot esculenta (Cassava) (Jatropha manihot); NCBI TaxID: 3983;
ModificationListTMT6plex; Oxidation; Carbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-24 00:02:51ID requested
12019-07-08 08:22:26announced
Publication List
Mehdi R, Lamm CE, Bodampalli Anjanappa R, M, ü, dsam C, Saeed M, Klima J, Kraner ME, Ludewig F, Knoblauch M, Gruissem W, Sonnewald U, Zierer W, Symplasmic phloem unloading and radial post-phloem transport via vascular rays in tuberous roots of Manihot esculenta. J Exp Bot, 70(20):5559-5573(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Cassava, Phloem, Symplast, Apoplast, SUC2, Esculin, CFDA, Ray, Morphology, Starch
Contact List
Wolfgang Zierer
contact affiliationDivision of Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstraße 5, 91058 Erlangen
contact emailWolfgang.Zierer@fau.de
lab head
Christian Lamm
contact affiliationFriedrich-Alexander Universität Erlangen-Nürnberg
contact emailChristian.lamm@fau.de
dataset submitter
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Dataset FTP location
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