PXD014001

PXD014001 is an original dataset announced via ProteomeXchange.

Title	Vascular rays mediate radial assimilate transport in tuberous roots of Manihot esculenta
Description	Cassava (Manihot esculenta) is one of the most important staple food crops worldwide. Its starchy tuberous roots supply over 800 million people with carbohydrates. Yet, surprisingly little is known about the processes involved in filling of those vital storage organs. A better understanding of cassava carbohydrate allocation and starch storage is key to improve storage root yield. In this work, we studied cassava morphology and phloem sap flow from source to sink using transgenic pAtSUC2::GFP plants, the phloem tracers esculin and 5(6)-carboxyfluorescein diacetate (CFDA), as well as several staining techniques. We show that cassava performs apoplasmic phloem loading in source leaves and symplasmic unloading into phloem parenchyma cells of tuberous roots. We demonstrate that vascular rays play an important role in radial transport from the phloem to xylem parenchyma cells in tuberous roots. Furthermore, enzymatic and proteomic measurements of storage root tissues confirmed high abundance and activity of enzymes involved in the sucrose synthase-mediated pathway and indicated that starch is stored most efficiently in the outer xylem layers of tuberous roots. Our findings represent a first basis for biotechnological approaches aimed at improved phloem loading and enhanced carbohydrate allocation and storage in order to increase tuberous root yield of cassava.
HostingRepository	PRIDE
AnnounceDate	2019-07-08
AnnouncementXML	Submission_2019-07-08_08:22:24.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014001
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Lamm
SpeciesList	scientific name: Manihot esculenta (Cassava) (Jatropha manihot); NCBI TaxID: 3983;
ModificationList	TMT6plex; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-24 00:02:51	ID requested
⏵ 1	2019-07-08 08:22:26	announced

Mehdi R, Lamm CE, Bodampalli Anjanappa R, M, ü, dsam C, Saeed M, Klima J, Kraner ME, Ludewig F, Knoblauch M, Gruissem W, Sonnewald U, Zierer W, Symplasmic phloem unloading and radial post-phloem transport via vascular rays in tuberous roots of Manihot esculenta. J Exp Bot, 70(20):5559-5573(2019) [pubmed]

curator keyword: Biological

submitter keyword: Cassava, Phloem, Symplast, Apoplast, SUC2, Esculin, CFDA, Ray, Morphology, Starch

Wolfgang Zierer
contact affiliation	Division of Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstraße 5, 91058 Erlangen
contact email	Wolfgang.Zierer@fau.de
lab head
Christian Lamm
contact affiliation	Friedrich-Alexander Universität Erlangen-Nürnberg
contact email	Christian.lamm@fau.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014001
     1. Label: PRIDE project
     2. Name: Vascular rays mediate radial assimilate transport in tuberous roots of Manihot esculenta