PXD013998

PXD013998 is an original dataset announced via ProteomeXchange.

Title	Analysis of Human Membrane Protein Complexes by Using Formaldehyde Cross-linking and Protein Correlation Profiling
Description	Membrane proteins are essential to many biological processes and account for two-thirds of the successful drug targets. Studying the membrane-protein complexes assembly will help to understand their biological functions comprehensively. We applied an approach that combines cross-linking mass spectrometry and protein correlation profiling to analysis the endogenous human membrane protein complexes. Finally, we constructed a network of 312 complexes involves 31,095 high-confident interactions with 2,333 proteins. These protein complexes are linked to core biological processes such as translation, intracellular transport, metabolic, and catabolic. They are also related to more than one hundred of human diseases, especially Alzheimer's disease, Carcinoma, and Endocarditis. Strikingly, 939 proteins participate in the interaction between different organelles, including ER and mitochondria, plasma membrane and mitochondria, and ER and vesicles. Besides that, possible cross-linking peptides were obtained, which provide further evidence and detailed structural information for the predicted protein complexes. This membrane-protein associated complexes map might benefit disease research and provide a new perspective on organelle communication.
HostingRepository	iProX
AnnounceDate	2019-05-24
AnnouncementXML	Submission_2019-05-23_20:29:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bing Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-23 20:24:00	ID requested
⏵ 1	2019-05-23 20:29:24	announced

Dataset with its publication pending

submitter keyword: Membrane protein complex, Cross-linking, Protein correlation profiling, Formaldehyde, Disease, Organelles communication

Cuihong Wan
contact affiliation	Central China Normal University
contact email	ch_wan@mail.ccnu.edu.cn
lab head
Bing Wang
contact affiliation	Central China Normal University
contact email	635819601@qq.com
dataset submitter

iProX dataset URI