PXD013987

PXD013987 is an original dataset announced via ProteomeXchange.

Title	U2OS osteosarcoma cells LC-MSMS
Description	In order to identify the targets of miR-193a-5p in osteosarcoma U2OS cell line, we used a lentivirus-mediated expression system to overexpressing miR-193a precusor, miR-193a-5p target sequence and non-target sequence, respectively, in osteosarcoma cell line U2OS. A tandem mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-193a-5p-regulated proteins. order to identify the targets of miR-193a-5p, we used a lentivirus-mediated expression system to overexpressing miR-193a precusor, miR-193a-5p target sequence and non-target sequence, respectively, in osteosarcoma cell line U2OS. A tandem mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-193a-5p-regulated proteins.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_11:07:50.667.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianlin Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-23 07:27:24	ID requested
⏵ 1	2021-09-08 11:07:51	announced

Chen R, Ning Y, Zeng G, Zhou H, Zhou L, Xiao P, Li Z, Zhou J, The miR-193a-5p/NCX2/AKT axis promotes invasion and metastasis of osteosarcoma. J Cancer, 12(19):5903-5913(2021) [pubmed]

submitter keyword: Osteosarcoma, U2OS, LC-MSMS, miR-193a-5p

Jianlin Zhou
contact affiliation	College of Life Sciences, Hunan Normal University
contact email	jlzhou@hunnu.edu.cn
lab head
Jianlin Zhou
contact affiliation	Hunan Normal University
contact email	jlzhou@hunnu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD013987

PRIDE project URI

• PRIDE
  1. PXD013987
     1. Label: PRIDE project
     2. Name: U2OS osteosarcoma cells LC-MSMS