PXD013974

PXD013974 is an original dataset announced via ProteomeXchange.

Title	Hypoxia-induced reprogramming of the cardiac phenotype in American alligators (Alligator mississippiensis) revealed by quantitative proteomics
Description	Hypoxic exposure during development can have a profound influence on offspring physiology, including cardiac dysfunction, yet many reptile embryos naturally experience periods of hypoxia in buried nests. American alligators experimentally exposed to developmental hypoxia demonstrate morphological and functional changes to the heart that persist into later life stages; however, the molecular bases of these changes remain unknown. We tested if targeted and persistent changes in steady-state protein expression underlie this hypoxic heart phenotype, using isobaric tags for relative and absolute quantitation (iTRAQ) proteomics. Alligator eggs were reared under normoxia or 10% hypoxia, then either sampled (embryo) or returned to normoxia for 2 years (juvenile). Three salient findings emerge from the integrated analysis of the 145 differentially expressed proteins in hypoxia-reared animals: (1) significant protein-protein interaction networks were identified only in up-regulated, indicating that the effects of developmental hypoxia are stimulatory and directed; (2) the up-regulated proteins substantially enriched processes related to protein turnover, cellular organization, and metabolic pathways, supporting increased resource allocation towards building and maintaining a higher functioning heart; and (3) the juvenile cardiac proteome retained many of the signature changes observed in embryonic hearts, supporting long-term reprogramming of cardiac myocytes induced by hypoxia during critical periods of development.
HostingRepository	MassIVE
AnnounceDate	2021-01-06
AnnouncementXML	Submission_2021-01-06_06:59:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Alderman
SpeciesList	scientific name: Alligator mississippiensis; common name: American alligator; NCBI TaxID: 8496;
ModificationList	iTRAQ8plex reporter+balance reagent acylated residue; Carbamidomethyl
Instrument	instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-22 10:01:31	ID requested
⏵ 1	2021-01-06 06:59:48	announced

Alderman SL, Crossley DA, Elsey RM, Gillis TE, Hypoxia-induced reprogramming of the cardiac phenotype in American alligators (Alligator mississippiensis) revealed by quantitative proteomics. Sci Rep, 9(1):8592(2019) [pubmed]

Alderman SL, Crossley DA, Elsey RM, Gillis TE, Growing up gator: a proteomic perspective on cardiac maturation in an oviparous reptile, the American alligator (Alligator mississippiensis). J Comp Physiol B, 190(2):243-252(2020) [pubmed]

submitter keyword: heart, reptile, oxygen, iTRAQ, developmental programming, phenotypic plasticity

Sarah Alderman
contact affiliation	University of Guelph
contact email	alderman@uoguelph.ca
lab head
Sarah Alderman
contact affiliation	University of Guelph
contact email	alderman@uoguelph.ca
dataset submitter

MassIVE dataset URI

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