PXD013973

PXD013973 is an original dataset announced via ProteomeXchange.

Title	Cysteine alkylating methods in shotgun proteomics and their possible effects on methionine residues
Description	Neatly every proteomic protocol has a Cysteine reduction and alkylation step in it preceding the trypsin digestion. It needs to be done in order to remove the s-s bonds and prevent proteins from folding, thus, provide better access for trypsin. Among various reagents used for alkylation, iodoacetamide (IAM) seems to be the most common. Although, it has been shown that IAM can cause some undesired side reactions [Chernobrovkin et al., 2015; Muller et al., 2017]. In this work, we have compared the effect of four alkylating agents: iodoacetamide (IAM), 4-vinylpyridine (4-VP), chloroacetamide (CAM) and S-Methyl methanethiosulfonate (MMTS). In addition, we tried to check whether post-treatment with DTT (p-red) is reasonable in the case of irreversible alkylation.
HostingRepository	PRIDE
AnnounceDate	2020-10-28
AnnouncementXML	Submission_2020-10-28_06:38:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Irina Ilina
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-22 08:41:31	ID requested
⏵ 1	2020-10-28 06:38:12	announced

Kuznetsova KG, Levitsky LI, Pyatnitskiy MA, Ilina IY, Bubis JA, Solovyeva EM, Zgoda VG, Gorshkov MV, Moshkovskii SA, Cysteine alkylation methods in shotgun proteomics and their possible effects on methionine residues. J Proteomics, 231():104022(2021) [pubmed]

submitter keyword: proteomics, alkylation, cysteine, methionine

Sergei A. Moshkovskii
contact affiliation	Lab. Med. Proteomics Institute of Biomedical Chemistry Chair of Biochemistry, Medico-Biological Faculty Pirogov Russian National Research Medical University Moscow, Russia
contact email	smosh@mail.ru
lab head
Irina Ilina
contact affiliation	IBMC
contact email	irinailina.ibmc@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013973
     1. Label: PRIDE project
     2. Name: Cysteine alkylating methods in shotgun proteomics and their possible effects on methionine residues