PXD013968

PXD013968 is an original dataset announced via ProteomeXchange.

Title	Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention
Description	Triple negative breast cancer (TNBC) is an aggressive subtype of breast cancer with limited therapeutic opportunities. Recently, splicing factors have gained attention as potential targets for cancer treatment. We performed an RNAi screen targeting 244 individual splicing factors to systematically evaluate their role in TNBC cell proliferation. We identified nine splicing factors, including SNRPD2, SNRPD3 and NHP2L1, of which depletion inhibited proliferation in two TNBC cell lines by deregulation of sister chromatid cohesion (SCC) via increased sororin intron 1 retention and down-regulation of SMC1, MAU2 and ESPL1. Protein-protein interaction analysis of SNRPD2, SNRPD3 and NHP2L1 identified that seven out of the nine identified splicing factors belong to the same spliceosome complex including novel componentSUN2 that was also critical for efficient sororin splicing. Finally, sororin transcript levels are highly correlated to various proliferation markers in BC patients, suggesting that deregulating sororin levels through targeting of the relevant splicing factors might be a potential strategy to treat TNBC
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:19:44.900.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Román González-Prieto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-22 03:13:49	ID requested
1	2021-03-07 14:52:28	announced
2	2021-03-07 22:41:09	announced	2021-03-08: Updated publication reference for PubMed record(s): 33648524.
⏵ 3	2024-10-22 05:19:48	announced	2024-10-22: Updated project metadata.

10.1186/s13046-021-01863-4;

Koedoot E, van Steijn E, Vermeer M, Gonz, á, lez-Prieto R, Vertegaal ACO, Martens JWM, Le D, é, v, é, dec SE, van de Water B, Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention. J Exp Clin Cancer Res, 40(1):82(2021) [pubmed]

submitter keyword: splicing,Triple-negative breast cancer

Alfred C.O. Vertegaal
contact affiliation	Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands.
contact email	a.c.o.vertegaal@lumc.nl
lab head
Román González-Prieto
contact affiliation	Andalusian Center for Regenerative Medicine and Cell Biology, CABIMER, University of Seville
contact email	R.Gonzalez_Prieto@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD013968

PRIDE project URI

• PRIDE
  1. PXD013968
     1. Label: PRIDE project
     2. Name: Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention