PXD013962

PXD013962 is an original dataset announced via ProteomeXchange.

Title	Proteome of proline betaine-grown Eubacterium limosum ATCC 8486
Description	Proline betaine, and to a much lesser extent, N-methyl L-proline, are found in citrus fruits and often present in the human metabolome. However, most of the N-methyl L-proline found in an animal model metabolome is produced by the microbiota, yet organisms and enzymes capable of producing N-methyl proline in the anoxic intestine have been unknown. The anaerobe Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. We found that this organism demethylates proline betaine and excretes N-methyl proline during growth. The proteome of proline betaine-grown Eubacterium limosum was obtained in order to identify enzymes required for growth on proline, in particular to identify components that are unique to growth on proline in comparison to other substrates for acetogenesis, such as lactic acid. Comparison of the proteomes of the bacteria on proline betaine and lactic acid led to identification of the proteins involved in a proline betaine:tetrahydrofolate methyltransferase system which was biochemically verified. The key proline betaine demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily.
HostingRepository	PRIDE
AnnounceDate	2019-07-25
AnnouncementXML	Submission_2019-07-25_08:28:15.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013962
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joseph Krzycki
SpeciesList	scientific name: Eubacterium limosum; NCBI TaxID: 1736;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-22 01:49:40	ID requested
⏵ 1	2019-07-25 08:28:15	announced

Dataset with its publication pending

submitter keyword: Acetogenic bacteria, human gut isolate, proline betaine, N-methyl proline

Joseph Adrian Krzycki
contact affiliation	The Ohio State University Department of Microbiology
contact email	Krzycki.1@osu.edu
lab head
Joseph Krzycki
contact affiliation	The Ohio State University
contact email	Krzycki.1@osu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013962
     1. Label: PRIDE project
     2. Name: Proteome of proline betaine-grown Eubacterium limosum ATCC 8486