PXD013947

PXD013947 is an original dataset announced via ProteomeXchange.

Title	Structural basis of TFIIH activation for nucleotide excision repair
Description	Nucleotide excision repair (NER) is the major DNA repair pathway that removes UV-induced and bulky DNA lesions. There is currently no structure of NER intermediates, which form around the large multisubunit transcription factor IIH (TFIIH). Here we report the cryo-EM structure of an NER intermediate containing TFIIH and the NER factor XPA. Compared to its transcription conformation, the TFIIH structure is rearranged such that its ATPase subunits XPB and XPD bind double- and single-stranded DNA, consistent with their translocase and helicase activities, respectively. XPA releases the inhibitory kinase module of TFIIH, displaces a ‘plug’ element from the DNA-binding pore in XPD, and together with the NER factor XPG stimulates XPD activity. Our results explain how TFIIH is switched from a transcription to a repair factor, and provide the basis for a mechanistic analysis of the NER pathway.
HostingRepository	PRIDE
AnnounceDate	2019-07-02
AnnouncementXML	Submission_2019-07-03_00:19:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandar Chernev
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-21 00:06:31	ID requested
1	2019-07-02 06:08:13	announced
⏵ 2	2019-07-03 00:19:54	announced	Updated publication reference for PubMed record(s): 31253769.

Kokic G, Chernev A, Tegunov D, Dienemann C, Urlaub H, Cramer P, Structural basis of TFIIH activation for nucleotide excision repair. Nat Commun, 10(1):2885(2019) [pubmed]

curator keyword: Biological

submitter keyword: BS3 cross-linking, NER pathway

Patrick Cramer
contact affiliation	Max Planck Institute for Biophysical Chemistry, Department of Molecular Biology
contact email	patrick.cramer@mpibpc.mpg.de
lab head
Aleksandar Chernev
contact affiliation	MPIbpc
contact email	aleksandar.chernev@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD013947

PRIDE project URI

• PRIDE
  1. PXD013947
     1. Label: PRIDE project
     2. Name: Structural basis of TFIIH activation for nucleotide excision repair