PXD013947 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Structural basis of TFIIH activation for nucleotide excision repair |
Description | Nucleotide excision repair (NER) is the major DNA repair pathway that removes UV-induced and bulky DNA lesions. There is currently no structure of NER intermediates, which form around the large multisubunit transcription factor IIH (TFIIH). Here we report the cryo-EM structure of an NER intermediate containing TFIIH and the NER factor XPA. Compared to its transcription conformation, the TFIIH structure is rearranged such that its ATPase subunits XPB and XPD bind double- and single-stranded DNA, consistent with their translocase and helicase activities, respectively. XPA releases the inhibitory kinase module of TFIIH, displaces a ‘plug’ element from the DNA-binding pore in XPD, and together with the NER factor XPG stimulates XPD activity. Our results explain how TFIIH is switched from a transcription to a repair factor, and provide the basis for a mechanistic analysis of the NER pathway. |
HostingRepository | PRIDE |
AnnounceDate | 2019-07-02 |
AnnouncementXML | Submission_2019-07-03_00:19:53.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Aleksandar Chernev |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-21 00:06:31 | ID requested | |
1 | 2019-07-02 06:08:13 | announced | |
⏵ 2 | 2019-07-03 00:19:54 | announced | Updated publication reference for PubMed record(s): 31253769. |
Publication List
Kokic G, Chernev A, Tegunov D, Dienemann C, Urlaub H, Cramer P, Structural basis of TFIIH activation for nucleotide excision repair. Nat Commun, 10(1):2885(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: BS3 cross-linking, NER pathway |
Contact List
Patrick Cramer |
contact affiliation | Max Planck Institute for Biophysical Chemistry, Department of Molecular Biology |
contact email | patrick.cramer@mpibpc.mpg.de |
lab head | |
Aleksandar Chernev |
contact affiliation | MPIbpc |
contact email | aleksandar.chernev@mpibpc.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013947
- Label: PRIDE project
- Name: Structural basis of TFIIH activation for nucleotide excision repair