PXD013939 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport |
Description | The kinesin-3 KIF1C is a fast organelle transporter implicated in the transport of dense core vesicles in neurons and the delivery of integrins to cell adhesions. Here we report the mechanisms of autoinhibition and release that control the activity of KIF1C. We show that the microtubule binding surface of KIF1C motor domain interacts with its stalk and that these autoinhibitory interactions are released upon binding of protein tyrosine phosphatase PTPN21. The FERM domain of PTPN21 stimulates dense core vesicle transport in primary hippocampal neurons and rescues integrin trafficking in KIF1C-depleted cells. In vitro, human full-length KIF1C is a processive, plus-end directed motor. Its landing rate onto microtubules increases in the presence of either PTPN21 FERM domain or the cargo adapter Hook3 that binds the same region of KIF1C tail. This autoinhibition release mechanism allows cargo-activated transport and might enable motors to participate in bidirectional cargo transport without undertaking a tug-of-war. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:56:50.834.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hamdi Hussain |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-20 07:45:48 | ID requested | |
1 | 2019-06-24 07:38:48 | announced | |
2 | 2019-06-27 02:01:23 | announced | Updated publication reference for PubMed record(s): 31217419. |
⏵ 3 | 2024-10-22 04:56:52 | announced | 2024-10-22: Updated project metadata. |
Publication List
Siddiqui N, Zwetsloot AJ, Bachmann A, Roth D, Hussain H, Brandt J, Kaverina I, Straube A, PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport. Nat Commun, 10(1):2693(2019) [pubmed] |
10.1038/s41467-019-10644-9; |
Keyword List
submitter keyword: Autoinhibition, PTPN21, HOOK3,KIF1C |
Contact List
Anne Straube |
contact affiliation | Centre for Mechanochemical Cell Biology, University of Warwick, Coventry, CV4 7AL, UK |
contact email | anne@mechanochemistry.org |
lab head | |
Hamdi Hussain |
contact affiliation | University of Warwick |
contact email | hamdi.hussain@warwick.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013939
- Label: PRIDE project
- Name: PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport