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PXD013939

PXD013939 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport
DescriptionThe kinesin-3 KIF1C is a fast organelle transporter implicated in the transport of dense core vesicles in neurons and the delivery of integrins to cell adhesions. Here we report the mechanisms of autoinhibition and release that control the activity of KIF1C. We show that the microtubule binding surface of KIF1C motor domain interacts with its stalk and that these autoinhibitory interactions are released upon binding of protein tyrosine phosphatase PTPN21. The FERM domain of PTPN21 stimulates dense core vesicle transport in primary hippocampal neurons and rescues integrin trafficking in KIF1C-depleted cells. In vitro, human full-length KIF1C is a processive, plus-end directed motor. Its landing rate onto microtubules increases in the presence of either PTPN21 FERM domain or the cargo adapter Hook3 that binds the same region of KIF1C tail. This autoinhibition release mechanism allows cargo-activated transport and might enable motors to participate in bidirectional cargo transport without undertaking a tug-of-war.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:56:50.834.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHamdi Hussain
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-20 07:45:48ID requested
12019-06-24 07:38:48announced
22019-06-27 02:01:23announcedUpdated publication reference for PubMed record(s): 31217419.
32024-10-22 04:56:52announced2024-10-22: Updated project metadata.
Publication List
Siddiqui N, Zwetsloot AJ, Bachmann A, Roth D, Hussain H, Brandt J, Kaverina I, Straube A, PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport. Nat Commun, 10(1):2693(2019) [pubmed]
10.1038/s41467-019-10644-9;
Keyword List
submitter keyword: Autoinhibition, PTPN21, HOOK3,KIF1C
Contact List
Anne Straube
contact affiliationCentre for Mechanochemical Cell Biology, University of Warwick, Coventry, CV4 7AL, UK
contact emailanne@mechanochemistry.org
lab head
Hamdi Hussain
contact affiliationUniversity of Warwick
contact emailhamdi.hussain@warwick.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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