PXD013924

PXD013924 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiles during adventitious rooting of Eucalyptus species relevant to the cellulose industry (Induction)
Description	Adventitious rooting (AR) is an obligatory step for vegetative propagation of commercial woody species. Paper industries have interest in Eucalyptus globulus Labill and its hybrids due to low lignin and lipid contents, which facilitate cellulose extraction. However, this species and some of its hybrids are recalcitrant to rooting, often requiring exogenous auxin supply. Here we performed a comparative analysis of proteome changes during AR of E. globulus and the easy-to-root species Eucalyptus grandis W. Hill and the effects of exogenous auxin in different phases of the process (induction and formation), using a label-free quantification method. We identified 398 differentially abundant proteins, which were predicted to be involved in different biological pathways, mainly oxidative stress, energy metabolism and photosynthesis. Notable differences between species included proteins involved in oxidative stress, carbon and secondary metabolism. Exogenous auxin appeared to affect the availability of carbon sources and other phytohormones, besides cell cycle and microtubule- related proteins. Important players were also identified in each phase of AR. This is the first in depth analysis of protein changes during AR in Eucalyptus. The findings can be used in future studies to evaluate rooting competence in different genotypes and provide leads for AR improvement.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:06.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arthur Fett-Neto
SpeciesList	scientific name: Eucalyptus globulus (Blue gum); NCBI TaxID: 34317; scientific name: Eucalyptus grandis; NCBI TaxID: 71139;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-20 03:13:46	ID requested
1	2019-08-19 01:12:15	announced
2	2019-10-25 04:59:19	announced	2019-10-25: Updated publication reference for DOI(s): 10.1007/s11056-019-09728-7.
⏵ 3	2023-11-14 08:50:07	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: auxin,adventitious roots, gel-free proteomics, Eucalyptus, protein pattern

Arthur G. Fett-Neto
contact affiliation	Center for Biotechnology and Department of Botany, Federal University of Rio Grande do Sul, Porto Alegre, P.O. Box 15005, 91501-970, Brazil
contact email	fettneto@cbiot.ufrgs.br
lab head
Arthur Fett-Neto
contact affiliation	Center for Biotechnology, UFRGS
contact email	fettneto@cbiot.ufrgs.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013924
     1. Label: PRIDE project
     2. Name: Proteomic profiles during adventitious rooting of Eucalyptus species relevant to the cellulose industry (Induction)