PXD013915

PXD013915 is an original dataset announced via ProteomeXchange.

Title	Secretome of rat smooth muscle cells with stretch
Description	The extracellular matrix (ECM) is fundamental to cellular and tissue structural integrity and provides mechanical cues to initiate diverse biological functions1. The quality and quantity of ECM determines tissue stiffness and controls gene expression, cell fate, and cell cycle progression in various cell types. ECM-cell interactions are mediated by focal adhesions (FAs), the main hub for mechanotransduction, connecting ECM, integrins and cytoskeleton. In the blood vessels, an additional layer of mechanical cues derived from pulsatile blood flow and pressure play a pivotal role in homeostasis and disease development. However, how cells sense and integrate different mechanical cues to maintain the blood vessel wall is largely unknown. Vascular ECM is synthesized by SMCs from mid-gestation to early postnatal period and determines the material and mechanical properties of the blood vessels14. Owing to the long half-life of ECM, blood vessels are generally thought to stand life-long mechanical stress without regeneration, particularly in large arteries. However, it is not completely known whether local micro-remodeling system exists for the maintenance of the vessel wall. To search for a potential remodeling factor(s), we performed cyclic stretch experiments (1 Hz; 20% strain) using rat vascular SMCs for 20 hours (h) with or without Brefeldin A (BFA), a protein transporter inhibitor. Conditioned media (CM) were analyzed using quantitative mass spectrometry.
HostingRepository	jPOST
AnnounceDate	2020-03-18
AnnouncementXML	Submission_2022-09-18_02:38:49.932.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sumio Ohtsuki
SpeciesList	scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-19 18:47:50	ID requested
1	2020-03-17 21:06:55	announced
2	2020-09-18 00:46:36	announced	2020-09-18: Updated PubMed.
3	2021-02-04 16:49:55	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 02:38:50	announced	2022-09-18: Updated FTP location.

Yamashiro Y, Thang BQ, Ramirez K, Shin SJ, Kohata T, Ohata S, Nguyen TAV, Ohtsuki S, Nagayama K, Yanagisawa H, Matrix mechanotransduction mediated by thrombospondin-1/integrin/YAP in the vascular remodeling. Proc Natl Acad Sci U S A, 117(18):9896-9905(2020) [pubmed]

submitter keyword: rat, SMCs, stretch, secretome

Sumio Ohtsuki
lab head
Sumio Ohtsuki
contact affiliation	Kumamoto University
dataset submitter

jPOST dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000600/