PXD013908

PXD013908 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the early development of the Phalaenopsis amabilis flower bud under low temperature induction
Description	Phalaenopsis amabilis, one of the most important flowers in the current international flower market, is a plant that undergoes vernalization and requires low temperature treatment for flowering. There have been few reports on the proteomic analysis of the development of flower buds. In this study, by using isobaric tags for relative and absolute quantification (iTRAQ), 4096 differentially expressed proteins were identified in P. amabilis under low temperature treatment, of which 42 were associated with early floral induction, and 18 were verified by mass spectrometry multi-reaction monitoring (MRM). Among the proteins associated with the vernalization pathway, PEQU_11434 (glycine-rich RNA-binding protein GRP1A-like) and PEQU_11045 (UDP-N-acetylglucosamine diphosphorylase) were upregulated compared to their expression in control flower buds. It was therefore inferred that O-GlcNAc glycosylation was involved in the posttranscriptional modification of VRN1 (API homolog) and that the GRP2 protein (glycine-rich RNA-binding protein) was glycosylated to relieve binding to the VRN1 mRNA precursor to promote the expression of VRN1, which initiates floral development. Furthermore, phytochromes A (PEQU_13449, PEQU_35378), B (PEQU_09249) and C (PEQU_41401) were downregulated under low temperature treatment compared to their expression in control flower buds, suggesting that they could repress the expression of the VRN2 gene and thus release its repression of VRN3 to enable the high-level expression of FTPEQU_19304 (FT, VRN3 homolog), which promotes VRN1 expression and then stimulates flowering.
HostingRepository	PRIDE
AnnounceDate	2020-03-20
AnnouncementXML	Submission_2020-03-19_23:42:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cong Chen
SpeciesList	scientific name: Phalaenopsis amabilis; NCBI TaxID: 148545;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600+

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-17 08:41:19	ID requested
⏵ 1	2020-03-19 23:42:40	announced

Chen C, Zeng L, Zhao H, Ye Q, Flower Bud under Low Temperature Induction Using the iTRAQ/MRM Approach. Molecules, 25(5):(2020) [pubmed]

submitter keyword: Phalaenopsis amabilis

low temperature

flower bud induction

Qingsheng Ye
contact affiliation	Guangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China
contact email	ye-lab@scnu.edu.cn
lab head
Cong Chen
contact affiliation	Guangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China
contact email	39446004@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013908
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the early development of the Phalaenopsis amabilis flower bud under low temperature induction