PXD013906

PXD013906 is an original dataset announced via ProteomeXchange.

Title	CUL3(BPM) E3 ubiquitin ligases regulate MYC2, MYC3 and MYC4 stability and JA responses
Description	The Jasmonate pathway regulators MYC2, MYC3 and MYC4 are central nodes in plant signaling networks integrating environmental and developmental signals to fine-tune jasmonate defenses and plant growth. Hence, their activity needs to be tightly regulated in order to optimize plant fitness. Among the increasing number of mechanisms regulating MYCs, protein stability is arising as a major player. However, how the levels of MYCs proteins are modulated is still poorly understood. Here, we report that MYC2, MYC3 and MYC4 are targets of BPM proteins, which act as substrate adaptors of CUL3-based E3 ubiquitin ligases. Reduction-of-function of CUL3(BPM) in amiR-bpm lines, bpm235 triple mutants and cul3ab double mutants enhance MYC2 and MYC3 stability and accumulation, and potentiates plant responses to JA such a root-growth inhibition, and MYC-regulated gene expression. BPM3 protein is stabilized by JA, suggesting a new negative feedback regulatory mechanism to control MYCs activity. Our results uncover a new layer for JA-pathway regulation by CUL3(BPM)-mediated degradation of MYC TFs.
HostingRepository	PRIDE
AnnounceDate	2021-03-19
AnnouncementXML	Submission_2021-03-19_04:44:01.595.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-17 07:09:22	ID requested
⏵ 1	2021-03-19 04:44:02	announced

Chico JM, Lechner E, Fernandez-Barbero G, Canibano E, Garc, í, a-Casado G, Franco-Zorrilla JM, Hammann P, Zamarre, ñ, o AM, Garc, í, a-Mina JM, Rubio V, Genschik P, Solano R, E3 ubiquitin ligases regulate MYC2, MYC3, and MYC4 stability and JA responses. Proc Natl Acad Sci U S A, 117(11):6205-6215(2020) [pubmed]

submitter keyword: Jasmonate, MYC, phytohormone, proteasome, ubiquitination

Roberto Solano
contact affiliation	Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, Campus Universidad Autonoma, 28049 Madrid, Spain.
contact email	rsolano@cnb.csic.es
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013906
     1. Label: PRIDE project
     2. Name: CUL3(BPM) E3 ubiquitin ligases regulate MYC2, MYC3 and MYC4 stability and JA responses