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PXD013894

PXD013894 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe ER protein translocation channel subunit Sbh1 controls virulence of Cryptococcus neoformans
DescriptionC. neoformans is an opportunistic human pathogen whose polysaccharide capsule anchored to the cell wall is critical for virulence. Biogenesis of both cell wall and capsule relies on the secretory pathway. Protein secretion begins with protein translocation across the endoplasmic reticulum (ER) membrane through a highly conserved channel formed by three proteins, Sec61, Sbh1, and Sss1. Sbh1 is most divergent and contains multiple phosphorylation sites which may allow it to control entry into the secretory pathway in a regulated, species- and protein-specific manner. We show here that in contrast to S. cerevisiae, C. neoformans lacking SBH1 are barely temperature-sensitive, but that absence of SBH1 causes a cell-wall defect in both species. Comparison of the proteomes of wildtype and Δsbh1 C. neoformans revealed a small set of secretory and transmembrane proteins whose expression under infection-like conditions was upregulated in wildtype, but not in the Δsbh1 mutant. These proteins are mostly involved in cell-wall biogenesis. We found that adhesion to macrophages was compromised in the Δsbh1 strain and in mice, the C. neoformans Δsbh1 mutant was virtually avirulent. We conclude that upon contact with the host Sbh1 controls entry of virulencefactors into the secretory pathway of Cryptococcus neoformans.
HostingRepositoryPRIDE
AnnounceDate2023-01-27
AnnouncementXMLSubmission_2023-01-27_07:14:06.769.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJenniferGeddes-McAlister
SpeciesList scientific name: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii); NCBI TaxID: 235443;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-17 03:19:26ID requested
12023-01-27 07:14:07announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: secretion, proteomics,Cryptococcus neoformans, ER
Contact List
JenniferGeddes-McAlister
contact affiliationMolecular and Cellular Biology Department, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
contact emailjgeddesm@uoguelph.ca
lab head
JenniferGeddes-McAlister
contact affiliationUniversity of Guelph
contact emailjgeddesm@uoguelph.ca
dataset submitter
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Dataset FTP location
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