PXD013891

PXD013891 is an original dataset announced via ProteomeXchange.

Title	Meiosis-associated argonaute (MAGO) proteins are necessary for meiosis in maize
Description	Plants do not specify their germline until late in their life cycle. Hence, the plant germline is normally specified from terminally differentiated somatic cells, though the precise mechanism(s) are unknown. We have found that male gametogenesis in maize is associated with the accumulation of distinct 21-nt phased small-interfering RNAs (phasiRNAs) generated by meiosis-associated argonaute (MAGO) proteins. MAGO1 accumulates in the epidermis of pre-meiotic anthers while MAGO2 is found in developing meiocytes. We have found that MAGO proteins are required for meiocyte development as mutants display chromosomal defects and male infertility. Furthermore, we detect the heat stress-induced activation of a distinct class of Long terminal repeat retrotransposons in the male germline of MAGO mutants. Our data suggests that MAGO proteins and the reproductive phasiRNAs play important roles protecting the germline from transposable elements during environmental stress conditions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:23.224.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013891
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	Phospho; Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-17 01:36:28	ID requested
1	2022-01-04 02:43:00	announced
⏵ 2	2023-11-14 08:50:23	announced	2023-11-14: Updated project metadata.

10.6019/PXD013891;

submitter keyword: Zea mays, Meiosis, Maize,Argonaute, Proteomics, Phosphorylation

Dr Jose Gutierrez-Marcos
contact affiliation	School of Life Sciences, University of Warwick, Coventry, United Kingdom
contact email	J.F.Gutierrez-Marcos@warwick.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013891
     1. Label: PRIDE project
     2. Name: Meiosis-associated argonaute (MAGO) proteins are necessary for meiosis in maize