PXD013884

PXD013884 is an original dataset announced via ProteomeXchange.

Title	Desumoylation of the RNA polymerase III lies at the core of the Sumo-Stress Response
Description	Posttranslational modification by the small ubiquitin-like modifier Sumo regulates many cellular processes including the adaptive response to various types of stress, which has been referred to as Sumo Stress Response (SSR). However, it remains unclear whether the SSR involves a common set of core proteins regardless of the type of stress, or whether each particular type of stress induces a stress-specific SSR that targets a unique, largely non-overlapping set of Sumo substrates. In this study we used mass spectrometry to identify differentially sumoylated proteins during heat shock, hyperosmotic stress, oxidative stress, nitrogen starvation and DNA alkylation in Saccharomyces cerevisiae. Our results show that each stress triggers a specific SSR signature centered on proteins involved in transcription, translation, and chromatin regulation. Strikingly, while the various stress-specific SSRs were largely non-overlapping, all types of stresses tested here resulted in desumoylation of subunits of RNA polymerase III, which correlated with a decrease in tRNA synthesis. We conclude that desumoylation and subsequent inhibition of RNA polymerase III constitutes the core of all stress-specific SSRs.
HostingRepository	PRIDE
AnnounceDate	2019-11-01
AnnouncementXML	Submission_2019-11-08_00:38:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuula Nyman
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	sumoylated lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-16 06:51:38	ID requested
1	2019-11-01 05:20:43	announced
⏵ 2	2019-11-08 00:38:59	announced	2019-11-08: Updated publication reference for PubMed record(s): 31676685.

Ngu, é, a P A, Robertson J, Herrera MC, Chymkowitch P, Enserink JM, Desumoylation of RNA polymerase III lies at the core of the Sumo stress response in yeast. J Biol Chem, 294(49):18784-18795(2019) [pubmed]

curator keyword: Biological

submitter keyword: stress response, RNA polymerase III, small ubiquitin‐like modifier (SUMO), transcription regulation, transfer RNA (tRNA), Saccharomyces cerevisiae

Jorrit Enserink
contact affiliation	Department of Molecular Cell Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N-0379 Oslo, Norway
contact email	jorrit.enserink@rr-research.no
lab head
Tuula Nyman
contact affiliation	University of Oslo
contact email	tuula.nyman@medisin.uio.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013884
     1. Label: PRIDE project
     2. Name: Desumoylation of the RNA polymerase III lies at the core of the Sumo-Stress Response