PXD013869

PXD013869 is an original dataset announced via ProteomeXchange.

Title	Peptidomics analysis of dromedary camel supraoptic nucleus by tandem mass spectrometry
Description	Peptides were qualitatively characterized in supraoptic nuclei (SON)of dromedary camels by liquid chromatography-mass spectrometry/mass spectrometry. Samples collected in winter and summer were analyzed separately. Qualitative seasonal differences were noted. The presence of the PMCH hormone by detection of a single peptide, Neuropeptide-glutamic acid-isoleucine (EIGDEENSAKFPI-amide), only in winter SON. SCG1- and SCG2- derived peptides were detected in both seasons, but more peptides identified in winter than summer for either of the proteins. Peptides from SCG3 were detected only in winter SON samples. We found evidence of alternative splicing of the tachykinin precursor 1 in dromedary between seasons. In summer, we detected neurokinin A (isoforms 2,4, and 6) as well as peptide DADSSVEKQVALLKALYGLGQISHKMAYE confirming prohormone variant without neurokinin A (dromedary isoforms 3 and 7), while in winter SON we detected peptides supporting prohormone variants with neurokinin A (isoforms 2, 4, 6). Substance P was detected only in winter samples. The MS data supported some of our transcriptomics results.
HostingRepository	PRIDE
AnnounceDate	2019-11-11
AnnouncementXML	Submission_2019-11-11_15:00:23.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013869
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Elena Romanova
SpeciesList	scientific name: Camelus dromedarius; NCBI TaxID: 9838;
ModificationList	Phospho; Amidated; Glu->pyro-Glu; Oxidation; Acetyl; Dehydro; Gln->pyro-Glu
Instrument	impact HD

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-16 03:09:30	ID requested
⏵ 1	2019-11-11 15:00:25	announced

Alim FZD, Romanova EV, Tay YL, Rahman AYBA, Chan KG, Hong KW, Rogers M, Southey BR, Greenwood MP, Mecawi AS, Mustafa MR, Mahy N, Campbell C, Antunes-Rodrigues J, Sweedler JV, Murphy D, Hindmarch CCT, Seasonal adaptations of the hypothalamo-neurohypophyseal system of the dromedary camel. PLoS One, 14(6):e0216679(2019) [pubmed]

submitter keyword: supraoptic nucleus (SON), peptidomics, dehydration

Jonathan Sweedler
contact affiliation	Dept of Chemistry, University of Illinois at Urbana-Champaign
contact email	jsweedle@illinois.edu
lab head
Elena Romanova
contact affiliation	University of Illinois
contact email	romanova@illinois.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013869
     1. Label: PRIDE project
     2. Name: Peptidomics analysis of dromedary camel supraoptic nucleus by tandem mass spectrometry