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PXD013849 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRebuilding core abscisic acid signaling pathways of Arabidopsis in yeast
DescriptionThe signaling pathway of the phytohormone abscisic acid (ABA) regulates responses towards abiotic stress such as drought and high osmotic conditions. The multitude of functionally redundant components involved in ABA signaling, poses a major challenge for elucidating the largely unresolved response selectivity. We decided rebuilding single linear ABA signaling pathways in yeast for combinatoric permutation of ABA receptors and coreceptors, as well as the response-mediating SnRK2 protein kinases and their targeted transcription factors to drive luciferase expression in a heterologous host. We show that SnRK2s differ in the regulation by ABA receptor complexes, affect ABA responsiveness of the pathway, and differ in their transactivation activity but have similar preferences for ABA-responsive transcription factors. SnRK2s thought to act ABA-independently and known to be activated under osmotic stress in plants were regulated by ABA receptor complexes in yeast and competed with ABA receptor components in an ABA-dependent manner in plant tissue. The study reveals the suitability of the yeast system for analysis of ABA signaling factors and allowing the future dissection of ligand-receptor specificities in a functional response pathway. The analysis provides new insights into SnRK2 regulation indicating that four SnRK2 members of the osmotic stress response are tightly embedded into the ABA signaling pathway.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJulia Mergner
SpeciesList scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-14 10:28:34ID requested
12019-08-13 14:38:54announced
Publication List
Ruschhaupt M, Mergner J, Mucha S, Papacek M, Doch I, Tischer SV, Hemmler D, Chiasson D, Edel KH, Kudla J, Schmitt-Kopplin P, Kuster B, Grill E, Rebuilding core abscisic acid signaling pathways of Arabidopsis in yeast. EMBO J, 38(17):e101859(2019) [pubmed]
Keyword List
submitter keyword: SnRK2, ABA, phosphorylation
Contact List
Bernhard Kuster
contact affiliationTUM
contact emailkuster@tum.de
lab head
Julia Mergner
contact affiliationTUM
contact emailjulia.mergner@tum.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI