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PXD013833

PXD013833 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVitamin C-induced breast cancer cell death is associated with remodeling the redox state of cysteine-containing proteins involved in translation and cell cycle progression
DescriptionRecent studies show vitamin C (VitC) pro-oxidant properties at high pharmacological concentrations which favor repurposing VitC as an anti-cancer therapeutic agent. However, redox-based anticancer properties of different forms of VitC are yet partially understood. We examined the difference between the reduced and oxidized forms of VitC, ascorbic acid (AA) and dehydroascorbic acid (DHA), in terms of cytotoxicity and redox mechanisms toward breast cancer cells. Our data showed that AA displays higher cytotoxicity towards triple-negative breast cancer (TNBC) cell lines in vitro than DHA. AA has a similar cytotoxicity on non-TNBC breast cancer cells with much less effect on noncancerous cell lines. Using MDA-MB-231, a representative TNBC cell line, we observed that cellular redox-state alterations conditioned AA- and DHA-induced cytotoxicity. Hydrogen peroxide (H2O2) accumulation extracellularly and in different intracellular compartments, and to a less degree, induced intracellular GSH oxidation, played a key role in AA-induced cytotoxicity. In contrast, DHA affected GSH oxidation and thus had less cytotoxicity. Furthermore, different cytotoxicity is observed among breast cancer cell lines. Bioinformatics analysis and biological experiments showed that peroxiredoxin 1 (PRDX1) expression levels correlates with AA differential cytotoxicity in breast cancer cells. A “redoxome” proteomics approach revealed that AA treatment altered the redox state of key antioxidant enzymes (PRDX 1-3) and a number of cysteines-containing proteins including many nucleic acid binding proteins and proteins involved in RNA, DNA and energetic processes. We showed that cell cycle progression delay and translation inhibition are parts of the underlying mechanisms for AA-induced cytotoxicity.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:56:41.465.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThibaut LEGER
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListN-ethylmaleimide derivatized cysteine; biotinylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-14 02:12:58ID requested
12019-08-16 00:39:57announced
22024-10-22 04:56:42announced2024-10-22: Updated project metadata.
Publication List
El Banna N, Hatem E, Heneman-Masurel A, L, é, ger T, Ba, ï, lle D, Vernis L, Garcia C, Martineau S, Dupuy C, Vagner S, Camadro JM, Huang ME, Redox modifications of cysteine-containing proteins, cell cycle arrest and translation inhibition: Involvement in vitamin C-induced breast cancer cell death. Redox Biol, 26():101290(2019) [pubmed]
10.1016/j.redox.2019.101290;
Keyword List
submitter keyword: breast cancer, ascorbic acid,Vitamin C, dehydroascorbic acid, oxidative stress, redoxome
Contact List
Meng-Er Huang
contact affiliationCNRS UMR3348, Institut Curie, Bâtiment 110, Centre Universitaire, 91405 Orsay, France. Phone: 33-1-69863016; Fax: 33-1-69869429
contact emailmeng-er.huang@curie.fr
lab head
Thibaut LEGER
contact affiliationEHESP/LERES
contact emailthibaut.leger@ehesp.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
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