PXD013832

PXD013832 is an original dataset announced via ProteomeXchange.

Title	Comprehensive cell surface proteomics defines markers of classical, intermediate and non-classical monocytes
Description	Monocytes are a critical component of the cellular innate immune system, and can be subdivided into classical, intermediate and non-classical subsets on the basis of surface CD14 and CD16 expression. Classical monocytes play the canonical role of phagocytosis, and account for the majority of circulating cells. Intermediate and non-classical cells are known to exhibit varying levels of phagocytosis and cytokine secretion, and are differentially expanded in certain pathological states. Characterisation of cell surface proteins expressed by each subset is informative not only to improve understanding of phenotype, but also to provide biological insight into function. Here we use highly multiplexed Tandem-Mass-Tag (TMT)-based mass spectrometry with selective cell surface biotinylation to characterise the classical monocyte surface proteome, then interrogate the phenotypic differences between each monocyte subset to identify novel protein markers.
HostingRepository	PRIDE
AnnounceDate	2020-03-03
AnnouncementXML	Submission_2020-05-27_04:33:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Weekes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-14 02:12:32	ID requested
1	2020-03-02 23:13:57	announced
⏵ 2	2020-05-27 04:33:57	announced	2020-05-27: Updated publication reference for PubMed record(s): 32165698.

Ravenhill BJ, Soday L, Houghton J, Antrobus R, Weekes MP, Comprehensive cell surface proteomics defines markers of classical, intermediate and non-classical monocytes. Sci Rep, 10(1):4560(2020) [pubmed]

submitter keyword: Monocyte, blood cell, quantitative proteomics, tandem mass tag, cell surface, plasma membrane, classical monocyte

Michael Weekes
contact affiliation	Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK
contact email	mpw1001@cam.ac.uk
lab head
Michael Weekes
contact affiliation	University of Cambridge
contact email	mpw1001@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD013832

PRIDE project URI

• PRIDE
  1. PXD013832
     1. Label: PRIDE project
     2. Name: Comprehensive cell surface proteomics defines markers of classical, intermediate and non-classical monocytes