PXD013805

PXD013805 is an original dataset announced via ProteomeXchange.

Title	Antagonistic interactions between benzo[a]pyrene and C60 in toxicological response of Marine Mussel
Description	his integrated study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into mussel tissues was confirmed by GC-MS, LC-HRMS and ICP-MS. Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the toxic effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were down-regulated (~52 %). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1 % FDR). Using DEPs identified at a threshold of (p < 0.05; B[a]P and B[a]P mixture with nC60), gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that these proteins were enriched with a broad spectrum of biological processes and pathways, including those broadly associated with protein processing, cellular processes and environmental information processing. Among those significantly enriched pathways, the ribosome was consistently the top enriched term irrespective of treatment or concentration and plays an important role as the site of biological protein synthesis and translation. Our results demonstrate the complex multi-modal response to environmental stressors in M. galloprovincialis.
HostingRepository	PRIDE
AnnounceDate	2019-07-18
AnnouncementXML	Submission_2019-07-18_07:25:54.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013805
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laura Langan
SpeciesList	scientific name: Mytilus galloprovincialis; NCBI TaxID: 29158;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-13 05:21:20	ID requested
⏵ 1	2019-07-18 07:25:55	announced

Barranger A, Langan LM, Sharma V, Rance GA, Aminot Y, Weston NJ, Akcha F, Moore MN, Arlt VM, Khlobystov AN, Readman JW, Jha AN, ) in Toxicological Response of Marine Mussels. Nanomaterials (Basel), 9(7):(2019) [pubmed]

submitter keyword: Trojan Horse effect, B[a]P, nC60, co-exposure, mussels, DNA damage, proteomics

Awadhesh N. Jha
contact affiliation	Professor of Genetic Toxicology and Ecotoxicology School of Biological and Marine Science Plymouth University Plymouth United Kingdom
contact email	a.jha@plymouth.ac.uk
lab head
Laura Langan
contact affiliation	Baylor University
contact email	LAURA_LANGAN@BAYLOR.EDU
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013805
     1. Label: PRIDE project
     2. Name: Antagonistic interactions between benzo[a]pyrene and C60 in toxicological response of Marine Mussel