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PXD013801

PXD013801 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCD22 interactome .
DescriptionCD22 (Siglec-2) is a member of the Siglec family. It is an inhibitory co-receptor of the B-cell-receptor (BCR) and inhibits B–cell activation. Upon BCR stimulation ITIMs in the cytoplasmic tail of CD22 are phosphorylated. This triggers CD22 signalling pathways, which lead to a decreased calcium mobilization in the B cell and thus an inhibition of BCR signalling. Although some CD22 binding partners, such as the phosphatase SHP-1, have already been identified, we deciphered the CD22 interactome in more detail, to gain a deeper understanding of CD22 molecular mechanisms and signalling events after BCR activation. Stable isotope labelling of amino acids in cell culture (SILAC) in combination with mass spectrometry analysis enabled the identification of specific CD22 interaction partners in a quantitative proteomics approach. Hereby, several new CD22 associated proteins were identified that have not been linked to CD22 yet. One of those interacting proteins is cullin 3, an E3 ubiquitin ligase. It was revealed that cullin 3 is important for clathrin-dependent CD22 internalization after BCR stimulation and CD22 surface expression. Further analysis of B-cell specific cullin 3 deficient mice showed an important role of cullin 3 in B cell development. These mice have strongly reduced numbers of mature B cells in the periphery, which are characterized by increased CD22 expression and additionally by pre-activated and apoptotic phenotypes.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:27:06.865.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFriedel Drepper
SpeciesList scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-13 00:34:52ID requested
12024-10-07 10:27:07announced
Publication List
Meyer SJ, B, ö, ser A, Korn MA, Koller C, Bertocci B, Reimann L, Warscheid B, Nitschke L, Cullin 3 Is Crucial for Pro-B Cell Proliferation, Interacts with CD22, and Controls CD22 Internalization on B Cells. J Immunol, 204(12):3360-3374(2020) [pubmed]
10.4049/jimmunol.1900925;
Keyword List
curator keyword: Biological
submitter keyword: cullin 3, SILAC, B cells,CD22, QExactive Plus, DT40 cells
Contact List
Bettina Warscheid
contact affiliationDepartment of Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology and Signalling Research Centres BIOSS and CIBSS, University of Freiburg, 79104 Freiburg im Breisgau, Germany
contact emailbettina.warscheid@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliationAG Warscheid Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg Germany
contact emailfriedel.drepper@biologie.uni-freiburg.de
dataset submitter
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Dataset FTP location
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