PXD013798

PXD013798 is an original dataset announced via ProteomeXchange.

Title	endoplasmic reticulum stress-induced tRNA fragmentation
Description	Transfer RNA-derived fragments have specific biological roles. However, it is still not characterized what factors are responsible for generation of 5′-tRHs in certain conditions, such as metabolic disease and maturation of reproductive cells. Here, we report that Inositol-requiring enzyme 1α (IRE1α), a major ER stress sensor protein, cleaves specifically anticodon stem-loop region of tRNAGly(GCC) and produces 5′-tRHs. Using an RNA-seq-based approach, we identified cleavage sites in tRNAGly(GCC), generating 5′-tRHs in KGN cells (human ovarian granulosa cells) in an IRE1α-expression dependent manner. In vitro cleavage analyses further supported that IRE1α generates 5′-tRHs from tRNAGly(GCC) (5′-tRH-GlyGCC) with highly selective target discrimination. The production of 5′-tRH-GlyGCC was promoted upon endoplasmic reticulum (ER) stress, which induced IRE1α expression, in KGN cells as well as other cancer cell lines such as HeLa and HepG. In addition, transfection of synthetic 5′-tRH-GlyGCC mimics promoted survival of KGN and HeLa cells; this effect required expression of HNRNPM and HNRNPH2, which were identified as binding proteins of 5′-tRH-GlyGCC.
HostingRepository	PRIDE
AnnounceDate	2024-10-30
AnnouncementXML	Submission_2024-10-30_00:31:11.538.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong-Hak Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-10 07:19:35	ID requested
⏵ 1	2024-10-30 00:31:11	announced

10.1038/S41467-024-53624-4;

submitter keyword: HNRNPH2,IRE1α, ER stress, HNRNPM, tRNA fragment, tRNA-Gly(GCC)

Kim, Yong-Hak
contact affiliation	Daegu Catholic University School of Medicine
contact email	ykim@cu.ac.kr
lab head
Yong-Hak Kim
contact affiliation	Catholic University of Daegu School of Medicine
contact email	ykim@cu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013798
     1. Label: PRIDE project
     2. Name: endoplasmic reticulum stress-induced tRNA fragmentation