PXD013792

PXD013792 is an original dataset announced via ProteomeXchange.

Title	Proteins that interact physically with Drosophila blanks
Description	RNA interference targets aberrant transcripts with cognate small interfering RNAs, which derive from double-stranded RNA precursors through nucleolytic processing. Several functional screens have identified Drosophila blanks/lump (CG10630) as a facilitator of RNAi in cultured cells, yet its molecular function has remained unknown. The protein carries two dsRNA binding domains (dsRBD) and blanks mutant males have a spermatogenesis defect. We demonstrate that blanks selectively boosts RNAi triggered by dsRNA of nuclear origin. Blanks binds dsRNA in vitro, shuttles between nucleus and cytoplasm and the abundance of certain endogenous siRNAs is reduced in blanks mutant testes. Transgenic expression of a Blanks protein variant with increased nuclear retention reduces endogenous siRNA abundance unless the protein also carries a mutation that prevents binding of dsRNA to the second dsRBD. Blanks thus facilitates the export of dsRNA to the cytoplasm for further processing by the canonical RNAi machinery. In contrast, rescue of male fertility in blanks mutant animals was independent of RNA binding to the second dsRBD, consistent with a previous report that linked fertility to the first dsRBD of Blanks. The role of blanks in spermatogenesis appears thus unrelated to its role in dsRNA export.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-27_02:56:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Klaus Förstemann
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-10 00:56:19	ID requested
⏵ 1	2020-05-27 02:56:14	announced

Nitschko V, Kunzelmann S, Fr, ö, hlich T, Arnold GJ, F, ö, rstemann K, Trafficking of siRNA precursors by the dsRBD protein Blanks in Drosophila. Nucleic Acids Res, 48(7):3906-3921(2020) [pubmed]

curator keyword: Biological

submitter keyword: interaction with tagged blanks

Klaus Förstemann
contact affiliation	Department of Biochemistry and Gene Center, Ludwig-Maximilians-Universität München, Feodor-Lynen-strasse 25, 81377 München
contact email	foerstemann@genzentrum.lmu.de
lab head
Klaus Förstemann
contact affiliation	Ludwig-Maximilians-Universität München
contact email	foerstemann@genzentrum.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD013792

PRIDE project URI

• PRIDE
  1. PXD013792
     1. Label: PRIDE project
     2. Name: Proteins that interact physically with Drosophila blanks