PXD013775

PXD013775 is an original dataset announced via ProteomeXchange.

Title	Chemical phosphoproteomic approaches reveal substrate specificity of phosphoprotein phosphatases-1 and -2
Description	The phosphatases PP1 and PP2A are responsible for the majority of dephosphorylation reactions on phosphoserine (pSer) and –threonine (pThr), and are involved in basically all cellular processes and many related diseases. They are thought to have no appreciable intrinsic substrate specificity, but to gain specificity only in their holoenzyme forms. Through the development of a peptide library approach and application of a complementary phosphoproteomics assay, we uncover that PP1 and PP2A show intrinsic specificity towards pThr over pSer, as well as toward the sequence context surrounding the phospho-site. Our data reveal that PP1 is a key phosphatase of the 14-3-3 protein binding motif. This result enabled us to establish a previously unknown role for PP1 as regulator of the GRB-associated-binding-protein 2 (Gab2)/14-3-3 complex, exemplifying predictive potential of the data. Thus, our work should serve as a rich resource for (de)phosphorylation studies covering multiple cellular processes and phosphoproteins.
HostingRepository	PRIDE
AnnounceDate	2020-07-14
AnnouncementXML	Submission_2020-08-11_04:33:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominic Helm
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-08 07:56:43	ID requested
1	2020-07-14 04:59:35	announced
⏵ 2	2020-08-11 04:33:54	announced	2020-08-11: Updated publication reference for PubMed record(s): 32681005.

Hoermann B, Kokot T, Helm D, Heinzlmeir S, Chojnacki JE, Schubert T, Ludwig C, Berteotti A, Kurzawa N, Kuster B, Savitski MM, K, ö, hn M, Dissecting the sequence determinants for dephosphorylation by the catalytic subunits of phosphatases PP1 and PP2A. Nat Commun, 11(1):3583(2020) [pubmed]

curator keyword: Biological

submitter keyword: Phospho peptide library, PP1, PP2A

Mikhail Savitski
contact affiliation	EMBL, Heidelberg
contact email	mikhail.savitski@embl.de
lab head
Dominic Helm
contact affiliation	EMBL PCF
contact email	dominic.helm@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD013775

PRIDE project URI

• PRIDE
  1. PXD013775
     1. Label: PRIDE project
     2. Name: Chemical phosphoproteomic approaches reveal substrate specificity of phosphoprotein phosphatases-1 and -2