PXD013769

PXD013769 is an original dataset announced via ProteomeXchange.

Title	Cross-linking mass spectrometry of the CCAN and CCAN-Cenp-A complexes
Description	Here we performed a cross-linking mass spectrometry analysis of the inner kinethocore complex CCAN and of the complex associated with a CenpA-nucleosome (CCAN-CenpA)from budding yeast. These experiments validated the cryo-EM structure of both complexes and provided additional structural insights on the complex organization.
HostingRepository	PRIDE
AnnounceDate	2023-05-15
AnnouncementXML	Submission_2023-05-15_02:24:27.834.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DomenicoFasci
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-08 04:26:33	ID requested
1	2019-08-20 04:47:42	announced
2	2019-10-08 01:33:40	announced	2019-10-08: Updated publication reference for PubMed record(s): 31578520.
⏵ 3	2023-05-15 02:24:28	announced	2023-05-15: Updated project metadata.

Yan K, Yang J, Zhang Z, McLaughlin SH, Chang L, Fasci D, Ehrenhofer-Murray AE, Heck AJR, Barford D, Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome. Nature, 574(7777):278-282(2019) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: S.Cerevisiae, cryo-EM structure,Inner kinetochore complex, XL-MS

Albert J.R.Heck
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
contact email	A.J.R.Heck@uu.nl
lab head
DomenicoFasci
contact affiliation	Utrecht University
contact email	d.fasci@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013769

PRIDE project URI

• PRIDE
  1. PXD013769
     1. Label: PRIDE project
     2. Name: Cross-linking mass spectrometry of the CCAN and CCAN-Cenp-A complexes