PXD013762
PXD013762 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | HUVEC mCherry or GPNMB-mChery over expressed, mCherry-IP |
Description | HUVEC were infected retroviral vectors expressing mCherry-GPNMB or mCherry. The cells were lysed with Pierce IP lysis buffer. The lysates after immunoprecipitation using anti-mCherry antibody (ab213511, Abcam) was transferred into a new microcentrifuge tube for “tube gel digestion”, a modified in-gel digestion procedure, to generate dithiothreitol-reduced iodoacetamide-alkylated tryptic peptides for nano-flow liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). |
HostingRepository | jPOST |
AnnounceDate | 2022-05-08 |
AnnouncementXML | Submission_2022-09-18_02:20:34.341.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Masayoshi Suda |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Gln); 2-pyrrolidone-5-carboxylic acid (Glu); L-methionine sulfoxide; unknown modification |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-05-08 01:18:20 | ID requested | |
1 | 2022-05-07 08:00:04 | announced | |
⏵ 2 | 2022-09-18 02:20:34 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: GPNMB, HUVEC |
Contact List
Tohru Minamino | |
---|---|
lab head | |
Masayoshi Suda | |
contact affiliation | Niigata University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000594/ |