PXD013761

PXD013761 is an original dataset announced via ProteomeXchange.

Title	The Interactome analysis of the Respiratory Syncytial Virus protein M2-1 suggests a new role in viral mRNA metabolism post-transcription
Description	Human respiratory syncytial virus (RSV) is a globally prevalent negative-stranded RNA virus, which can cause life-threatening respiratory infections in young children, elderly people and immunocompromised patients. Its transcription termination factor M2-1 plays an essential role in viral transcription, but the mechanisms underpinning its function are still unclear. We investigated the cellular interactome of M2-1 using green fluorescent protein (GFP)-trap immunoprecipitation on RSV infected cells coupled with mass spectrometry analysis. We identified 137 potential cellular binding partners of M2-1, among which many proteins associated either with translation or mRNA metabolism, and particularly mRNA maturation and stabilization. Specific immunoprecipitation using the GFP-trap was used to confirm the ability of the polyA-binding protein cytoplasmic 1 (PABPC1), a candidate with a major role in both cellular translation and mRNA stabilization, to interact individually with M2-1. PABPC1 was also shown to colocalize with M2-1 from its accumulation in IBAGs to its liberation in the cytoplasm. These results strongly suggest a continued association of M2-1 with viral mRNA and mRNA metabolism factors from transcription to translation, and imply that M2-1 may have an additional role in the fate of viral mRNA downstream of transcription.
HostingRepository	PRIDE
AnnounceDate	2019-10-11
AnnouncementXML	Submission_2019-11-20_02:12:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut LEGER
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion ETD; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-07 09:10:07	ID requested
1	2019-10-11 00:36:15	announced
⏵ 2	2019-11-20 02:12:29	announced	2019-11-20: Updated publication reference for PubMed record(s): 31649314.

Bouillier C, Cosentino G, L, é, ger T, Rincheval V, Richard CA, Desquesnes A, Sitterlin D, Blouquit-Laye S, El, é, ou, ë, t JF, Gault E, Rameix-Welti MA, The Interactome analysis of the Respiratory Syncytial Virus protein M2-1 suggests a new role in viral mRNA metabolism post-transcription. Sci Rep, 9(1):15258(2019) [pubmed]

submitter keyword: Human, virus, RSV, AP-MS, M2-1, PABPC1

Marie-Anne Rameix-Welti
contact affiliation	UMR1173 INSERM, Université de Versailles St. Quentin, Montigny-le-Bretonneux, France
contact email	marie-anne.rameix-welti@uvsq.fr
lab head
Thibaut LEGER
contact affiliation	Institut Jacques Monod
contact email	thibaut.leger@ijm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013761
     1. Label: PRIDE project
     2. Name: The Interactome analysis of the Respiratory Syncytial Virus protein M2-1 suggests a new role in viral mRNA metabolism post-transcription