PXD013759 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Defining the NSD2 interactome: PARP1 PARylation reduces NSD2 histone methyltransferase activity and impedes chromatin binding |
Description | NSD2 is a histone methyltransferase that specifically dimethylates histone H3 lysine 36 (H3K36me2), a modification associated with gene activation. Dramatic overexpression of NSD2 in t(4;14) multiple myeloma (MM) and an activating mutation of NSD2 discovered in acute lymphoblastic leukemia (ALL) are significantly associated with altered gene activation, transcription and DNA damage repair. The partner proteins through which NSD2 may influence critical cellular processes remain poorly defined. In this study, we utilized proximity-based labelling (BioID) combined with label-free quantitative mass spectrometry to identify high confidence NSD2 interacting partners in MM cells. |
HostingRepository | PRIDE |
AnnounceDate | 2019-06-27 |
AnnouncementXML | Submission_2019-07-04_04:58:07.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD013759 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Xiaoxiao Huang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Deamidated; Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-07 08:25:43 | ID requested | |
1 | 2019-06-27 00:49:15 | announced | |
⏵ 2 | 2019-07-04 04:58:08 | announced | Updated publication reference for PubMed record(s): 31248990. |
Publication List
Huang X, LeDuc RD, Fornelli L, Schunter AJ, Bennett RL, Kelleher NL, Licht JD, Defining the NSD2 interactome: PARP1 PARylation reduces NSD2 histone methyltransferase activity and impedes chromatin binding. J Biol Chem, 294(33):12459-12471(2019) [pubmed] |
Keyword List
submitter keyword: NSD2, BioID, interacting partners, LC-MS/MS, label-free proteomics, multiple myeloma |
Contact List
Neil L Kelleher |
contact affiliation | Department of Chemistry, Department of Molecular Biosciences, and the Chemistry of Life Processes Institute, Northwestern University, Evanston, IL USA 60208 |
contact email | n-kelleher@northwestern.edu |
lab head | |
Xiaoxiao Huang |
contact affiliation | Northwestern University |
contact email | xiaoxiaohuang2012@u.northwestern.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD013759
- Label: PRIDE project
- Name: Defining the NSD2 interactome: PARP1 PARylation reduces NSD2 histone methyltransferase activity and impedes chromatin binding