PXD013759

PXD013759 is an original dataset announced via ProteomeXchange.

Title	Defining the NSD2 interactome: PARP1 PARylation reduces NSD2 histone methyltransferase activity and impedes chromatin binding
Description	NSD2 is a histone methyltransferase that specifically dimethylates histone H3 lysine 36 (H3K36me2), a modification associated with gene activation. Dramatic overexpression of NSD2 in t(4;14) multiple myeloma (MM) and an activating mutation of NSD2 discovered in acute lymphoblastic leukemia (ALL) are significantly associated with altered gene activation, transcription and DNA damage repair. The partner proteins through which NSD2 may influence critical cellular processes remain poorly defined. In this study, we utilized proximity-based labelling (BioID) combined with label-free quantitative mass spectrometry to identify high confidence NSD2 interacting partners in MM cells.
HostingRepository	PRIDE
AnnounceDate	2019-06-27
AnnouncementXML	Submission_2019-07-04_04:58:07.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013759
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Xiaoxiao Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-07 08:25:43	ID requested
1	2019-06-27 00:49:15	announced
⏵ 2	2019-07-04 04:58:08	announced	Updated publication reference for PubMed record(s): 31248990.

Huang X, LeDuc RD, Fornelli L, Schunter AJ, Bennett RL, Kelleher NL, Licht JD, Defining the NSD2 interactome: PARP1 PARylation reduces NSD2 histone methyltransferase activity and impedes chromatin binding. J Biol Chem, 294(33):12459-12471(2019) [pubmed]

submitter keyword: NSD2, BioID, interacting partners, LC-MS/MS, label-free proteomics, multiple myeloma

Neil L Kelleher
contact affiliation	Department of Chemistry, Department of Molecular Biosciences, and the Chemistry of Life Processes Institute, Northwestern University, Evanston, IL USA 60208
contact email	n-kelleher@northwestern.edu
lab head
Xiaoxiao Huang
contact affiliation	Northwestern University
contact email	xiaoxiaohuang2012@u.northwestern.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD013759

PRIDE project URI

• PRIDE
  1. PXD013759
     1. Label: PRIDE project
     2. Name: Defining the NSD2 interactome: PARP1 PARylation reduces NSD2 histone methyltransferase activity and impedes chromatin binding