PXD013758 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TFEB Immunoprecipitation And Proteome |
Description | Our studies in Salmonella Typhimurium (S. Typhimurium)-infected cells indicate that TFEB has a non-transcriptional, cytosolic function in addition to its well-characterized role as a transcription factor in regulating autophagy and lysosome biogenesis. An unbiased proteomics approach revealed that TFEB interacts with several mitochondrial proteins, which is lost when infected with S. Typhimurium. Microscopical and biochemical examinations further confirmed a novel localization of TFEB in mitochondria. We have also identified a TOMM20 binding motif within the protein sequence of TFEB which, facilitates the mitochondrial translocation in a mTOR-dependent manner. Our results further demonstrate that TFEB and the mitochondrial protease LonP1 co-regulate the assembly of complex I and its function. Moreover, during S. Typhimurium infection, lack of TFEB specifically in the mitochondria exacerbates the expression of pro-inflammatory cytokines. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:40:11.122.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hendrik Nolte |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-07 08:25:19 | ID requested | |
1 | 2024-05-21 10:02:01 | announced | |
⏵ 2 | 2024-10-22 06:40:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s44319-024-00058-0; |
Calabrese C, Nolte H, Pitman MR, Ganesan R, Lampe P, Laboy R, Ripa R, Fischer J, Polara R, Panda SK, Chipurupalli S, Gutierrez S, Thomas D, Pitson SM, Antebi A, Robinson N, Mitochondrial translocation of TFEB regulates complex I and inflammation. EMBO Rep, 25(2):704-724(2024) [pubmed] |
Keyword List
submitter keyword: IP, Proteome,TFEB, LC-MS/MS |
Contact List
Marcus Krüger |
contact affiliation | Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), 50931 Cologne, Germany. |
contact email | marcus.krueger@uni-koeln.de |
lab head | |
Hendrik Nolte |
contact affiliation | Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) |
contact email | h.nolte@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013758
- Label: PRIDE project
- Name: TFEB Immunoprecipitation And Proteome