PXD013755

PXD013755 is an original dataset announced via ProteomeXchange.

Title	Identification of NOS3 interacting proteins
Description	The endothelial nitric oxide (NO) synthase (eNOS, or NOS3) can be activated in response to fluid shear stress and numerous agonists via cellular events such as increased intracellular Ca2+, interaction with substrate, co-factors as well as adaptor and regulatory proteins, protein phosphorylation and S-glutathionylation in addition to shuttling between distinct sub-cellular domains. While some protein interactors modulate enzymatic activity, others can be S-nitrosation targets, which are brought in close proximity to the NO source under specific conditions. The identification of proteins interacting with eNOS in human endothelial cells stimulated with serum and growth factors may provide new insight into the regulation of eNOS activity as well as NO signaling via S-nitrosation.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_09:47:23.171.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-07 07:28:53	ID requested
⏵ 1	2021-09-08 09:47:24	announced

Siragusa M, Th, ö, le J, Bibli SI, Luck B, Loot AE, de Silva K, Wittig I, Heidler J, Stingl H, Randriamboavonjy V, Kohlstedt K, Br, ü, ne B, Weigert A, Fisslthaler B, Fleming I, Nitric oxide maintains endothelial redox homeostasis through PKM2 inhibition. EMBO J, 38(17):e100938(2019) [pubmed]

submitter keyword: LC-MSMS, proteome, vascular biology, eNOS

Ingrid Fleming
contact affiliation	Institute for Vascular Signalling, Centre for Molecular Medicine, Goethe University, Frankfurt am Main, Germany
contact email	fleming@vrc.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013755
     1. Label: PRIDE project
     2. Name: Identification of NOS3 interacting proteins