PXD013751

PXD013751 is an original dataset announced via ProteomeXchange.

Title	A closer look at Aspergillus: Online monitoring via scattered light enables reproducible phenotyping
Description	Development of microtiter plate based microbioreactor cultivation for Aspergillus giganteus with quasi-continuous online measurements. Different parameters such as well geometry, shaking frequency and morphology controlling agents were investigated in order to optimize the microtiter plate cultivation and scattered light signal towards reproducibility and homogeneity. An optimized medium was developed and scalability into stirred tank bioreactor cultivation was analyzed. As a transferability indicator the supernatant of both cultivation systems was analyzed for secreted protein patterns with a focus on an antifungal protein (AFP) and alpha-sarcin. These proteins were identified via LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2019-08-12
AnnouncementXML	Submission_2019-08-12_05:22:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bianca Klein
SpeciesList	scientific name: Aspergillus giganteus; NCBI TaxID: 5060;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-07 05:25:35	ID requested
⏵ 1	2019-08-12 05:22:20	announced

Jansen RP, Beuck C, Moch M, Klein B, K, ü, sters K, Morschett H, Wiechert W, Oldiges M, : online monitoring via scattered light enables reproducible phenotyping. Fungal Biol Biotechnol, 6():11(2019) [pubmed]

submitter keyword: Aspergillus giganteus

Microtiter plate

Miniaturized cultivation

Anti-fungal protein

High throughput bioprocess development

Morphology engineering

Marco Oldiges
contact affiliation	Forschungszentrum Juelich GmbH Institute of Bio- and Geosciences: Biotechnology 2 Wilhelm-Johnen-Str. 52425 Juelich Germany
contact email	m.oldiges@fz-juelich.de
lab head
Bianca Klein
contact affiliation	Forschungszentrum Juelich GmbH
contact email	b.klein@fz-juelich.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013751

PRIDE project URI

• PRIDE
  1. PXD013751
     1. Label: PRIDE project
     2. Name: A closer look at Aspergillus: Online monitoring via scattered light enables reproducible phenotyping