PXD013749

PXD013749 is an original dataset announced via ProteomeXchange.

Title	E. coli Affinity purification MS/MS
Description	The tRNA linked repeat sequence rrB was tagged in either 5'- or 3'- end with the MS2 aptamer and expressed from a plasmid in E. coli. Using the MS2 aptamer, the RNAs were affinity purified after cell lysis. The co-purifying proteins were identified by NanoLC-MS/MS and compared to the proteins co-purifying with the MS2 sequence alone.
HostingRepository	PRIDE
AnnounceDate	2021-04-26
AnnouncementXML	Submission_2021-04-25_21:30:48.255.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thoams Stenum
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-07 04:02:40	ID requested
⏵ 1	2021-04-25 21:30:48	announced

Dataset with its publication pending

submitter keyword: coli, MS2, affinity purification, RNA, TLR

Michael Askvad Sørensen
contact affiliation	University of Copenhagen, Department of biology (BIO) Ole Maaloe vej 5, 2200 Copenhagen N, Denmark
contact email	mas@bio.ku.dk
lab head
Thoams Stenum
contact affiliation	University of Copenhagen
contact email	thomasstenum@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD013749

PRIDE project URI

• PRIDE
  1. PXD013749
     1. Label: PRIDE project
     2. Name: E. coli Affinity purification MS/MS