PXD013725

PXD013725 is an original dataset announced via ProteomeXchange.

Title	Mechanism of antimicrobial synergy between doxycycline and carprofen against methicillin-resistant Staphylococus pseudintermedius
Description	We previously showed that doxycycline and carprofen , a veterinary non-steroidal anti-inflammatory drug, have synergistic antimicrobial activity against methicillin-resistant Staphylococus pseudintermedius (MRSP) carrying the tetracycline resistance determinant TetK. To elucidate the molecular mechanism of this synergy, we investigated the effects of the two drugs, individually and in combination, using a comprehensive approach including two-dimensional differential in-gel electrophoresis (2D DIGE).
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_13:59:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zofia Magnowska
SpeciesList	scientific name: Staphylococcus pseudintermedius; NCBI TaxID: 283734;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-07 02:41:56	ID requested
⏵ 1	2020-05-26 13:59:16	announced

Magnowska Z, Jana B, Brochmann RP, Hesketh A, Lametsch R, De Gobba C, Guardabassi L, Carprofen-induced depletion of proton motive force reverses TetK-mediated doxycycline resistance in methicillin-resistant Staphylococcus pseudintermedius. Sci Rep, 9(1):17834(2019) [pubmed]

submitter keyword: antimicrobial resistance, 2D DIGE

Luca Guardabassi
contact affiliation	Department of Veterinary and Animal Sciences University of Copenhagen Denmark
contact email	lg@sund.ku.dk
lab head
Zofia Magnowska
contact affiliation	University of Copenhagen
contact email	zofia@sund.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013725
     1. Label: PRIDE project
     2. Name: Mechanism of antimicrobial synergy between doxycycline and carprofen against methicillin-resistant Staphylococus pseudintermedius