PXD013724

PXD013724 is an original dataset announced via ProteomeXchange.

Title	Characterizing fruit ripening in plantain and Cavendish bananas: a proteomics approach
Description	The fruit physiology of banana cultivars is poorly understood. To study the ripening of plantain, a high-throughput proteomics experiment was executed and compared to the better known Cavendish. The starch content in plantain was at the onset of ripening very high and decreased to undetectable levels. Multivariate analysis of the proteins was performed correlated to the starch dynamics. The drop in sucrose synthase and the raise of acid invertase during ripening indicated a change in the balance of the sucrose fate. During ripening, sugars may no longer be available for respiration since they are stored in the vacuoles, making citrate the preferred respiratory substrate. One isoform of alfa amylase acted during the early phases of starch breakdown while another acted during the later phases when the starch branches are simpler in structure. We found significant variety specific differences in granule-bound starch synthase, alpha- and beta amylases, cell wall invertase and sucrose synthase that corroborate the difference in starch content/structure between both bananas. Differences in small heat shock proteins and in the cell wall-modifying enzyme xyloglucan endotransglucosylase/hydrolase support respectively the presumed higher carotenoid content and the firmer fruit structure of plantains.
HostingRepository	PRIDE
AnnounceDate	2020-06-08
AnnouncementXML	Submission_2020-06-07_22:58:55.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013724
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sebastien Carpentier
SpeciesList	scientific name: Musa acuminata subsp. acuminata; NCBI TaxID: 258441;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-07 02:35:41	ID requested
⏵ 1	2020-06-07 22:58:56	announced

Bhuiyan F, Campos NA, Swennen R, Carpentier S, Characterizing fruit ripening in plantain and Cavendish bananas: A proteomics approach. J Proteomics, 214():103632(2020) [pubmed]

submitter keyword: banana, plantain, fruit, ripening

Sebastien Carpentier
contact affiliation	SYBIOMA KULeuven
contact email	sebastien.carpentier@kuleuven.be
lab head
Sebastien Carpentier
contact affiliation	KULeuven
contact email	sebastien.carpentier@kuleuven.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD013724

PRIDE project URI

• PRIDE
  1. PXD013724
     1. Label: PRIDE project
     2. Name: Characterizing fruit ripening in plantain and Cavendish bananas: a proteomics approach