PXD013716

PXD013716 is an original dataset announced via ProteomeXchange.

Title	Protein changes in response to lead stress of lead-tolerant and lead-sensitive industrial hemp using SWATH technology
Description	Hemp is a Pb-tolerant and Pb-accumulating plant, and the study of its tolerance mechanisms could facilitate the breeding of hemp with enhanced Pb tolerance and accumulation. In the present study, we took advantage of SWATH (sequential window acquisition of all theoretical mass spectra) technology to study the difference in proteomics between the Pb-tolerant seed-type hemp variety Bamahuoma (BM) and the Pb-sensitive fiber-type hemp variety Yunma 1(Y1) under Pb stress (3g/kg soil). A total of 63 and 372 proteins differentially expressed under Pb stress relative to control conditions were identified with liquid chromatography electro spray ionization tandem mass spectrometry in BM and Y1, respectively; with each of these proteins being classified into 14 categories. Hemp adapted to Pb stress through accelerating ATP metabolism; enhancing respiration, light absorption and light energy transfer; promoting assimilation of intercellular nitrogen (N) and carbon (C); eliminating reactive oxygen species; regulating stomatal development and closure; improving exchange of water and CO2 in leaves; promoting intercellular transport; preventing aggregation of unfolded proteins; degrading misfolded proteins; and increasing the transmembrane transport of ATP in chloroplasts. Our results provide important reference protein and gene information for future molecular studies into the resistance and accumulation of Pb in hemp.
HostingRepository	iProX
AnnounceDate	2019-05-04
AnnouncementXML	Submission_2019-12-10_20:13:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Deng Gang
SpeciesList	scientific name: Eupatorium cannabinum; NCBI TaxID: 102770; scientific name: Iranian hemp witches'-broom phytoplasma; NCBI TaxID: 1005958;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-04 20:55:37	ID requested
1	2019-08-25 19:23:30	announced
⏵ 2	2019-12-10 20:13:06	announced	2019-12-11: Update publication information.

Xia C, Hong L, Yang Y, Yanping X, Xing H, Gang D, Protein Changes in Response to Lead Stress of Lead-Tolerant and Lead-Sensitive Industrial Hemp Using SWATH Technology. Genes (Basel), 10(5):(2019) [pubmed]

submitter keyword: Pb stress, Industrial hemp (Cannabis sativa L.), SWATH, Pb-stress adaption

Deng Gang
contact affiliation	Yunnan University
contact email	denggang1986@ynu.edu.cn
lab head
Deng Gang
contact affiliation	Yunnan University
contact email	denggang1986@ynu.edu.cn
dataset submitter

iProX dataset URI