PXD013710

PXD013710 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of urinary microvesicles reveal biomarkers for neurologic disease
Description	Background- Extracellular vesicles (EVs) harbor thousands of proteins that hold promise for biomarker development. Usually difficult to purify, EVs in urine are relatively easily obtained and have demonstrated efficacy for kidney disease prediction. Herein, we further characterize the proteome of urinary EVs to explore the potential for biomarkers unrelated to kidney dysfunction, focusing on Parkinson’s disease (PD). Methods- Using a quantitative mass spectrometry approach, we measured urinary EV proteins from a discovery cohort of 50 subjects. EVs in urine were classified into subgroups and EV proteins were ranked by abundance and variability over time. Enriched pathways and ontologies in stable EV proteins were identified and proteins that predict PD were further measured in a cohort of 108 subjects. Findings- Hundreds of commonly expressed urinary EV proteins with stable expression over time were distinguished from proteins expressed in few individual with high variability over time. Bioinformatic analyses reveal a striking enrichment of endolysosomal proteins linked to Parkinson’s, Alzheimer’s, Huntington’s disease, and other neurologic disorders. Tissue and biofluid enrichment analyses show broad representation of EVs from across the body without bias towards kidney or urine proteins. Among the proteins linked to neurological diseases, SNAP23 and calbindin were the most elevated in PD cases with 86% prediction success for disease diagnosis in the discovery cohort and 76% prediction success in the replication cohort.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-11_16:51:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013710
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	james mobley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-03 05:05:42	ID requested
⏵ 1	2019-11-11 16:51:06	announced

Wang S, Kojima K, Mobley JA, West AB, Proteomic analysis of urinary extracellular vesicles reveal biomarkers for neurologic disease. EBioMedicine, 45():351-361(2019) [pubmed]

submitter keyword: Biomarkers

Neurodegeneration

Exosomes

Parkinson's disease

Alzheimer's disease

James A. Mobley
contact affiliation	Director of the UAB CCC Mass Spectrometry Shared Facility
contact email	mobleyja@uab.edu
lab head
james mobley
contact affiliation	University of Alabama at Birmingham
contact email	mobleyja@uab.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013710
     1. Label: PRIDE project
     2. Name: Proteomic analysis of urinary microvesicles reveal biomarkers for neurologic disease