PXD013707

PXD013707 is an original dataset announced via ProteomeXchange.

Title	Deep proteome analysis of more than 12000 proteins in buffalo mammary epithelial cells identifies protein signatures for active proliferation and lactation
Description	Extensive branching morphogenesis takes place during pregnancy in the mammary gland. It is accompanied by the rapid proliferation of the Mammary Epithelial Cells (MECs). To gain insights into the proteomic changes that occur during the proliferation of MECs from buffalo (Bubalus bubalis) origin, we explored the deep proteome profile of buffalo mammary epithelial cells (BuMECs) using mass spectrometry (MS). To achieve this, we employed the sub-cellular fractionation approach and secretome analysis. Proteins were isolated separately from four subcellular fractions (SCFs) containing cytosolic (SCF-I), membranous and membranous organelle’s (SCF-II), nuclear (SCF-III) and cytoskeletal (SCF-IV). These sub-cellular specific protein fractions were processed using in-solution digestion and analyzed with nano-LCMS/MS. The MS analysis identified 8330, 5970, 5288 and 4818 non-redundant proteins in the fractions SCF I, II, III and IV respectively. To evaluate the secretory proteins in these cells, gel-based proteome approach was used which revealed a total of 792 non-redundant proteins. Altogether, combined analysis of all the five fractions including four sub-cellular fractions and secretome resulted in the identification of 12,609 non-redundant proteins. A total of 325 molecular pathways were identified after extensive analysis. The most enriched pathways associated with these proteins were metabolic, PI3-AKT, MAPK, mTOR, Insulin, estrogen and Oxytocin signaling. Our study demonstrated for the first time highest number of proteins identified by MS in any cell types including MECs.
HostingRepository	PRIDE
AnnounceDate	2020-03-20
AnnouncementXML	Submission_2020-03-20_00:56:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ashok Kumar Mohanty
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	carbamoylated residue; phosphorylated residue; monohydroxylated residue; iodoacetic acid derivatized residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-03 03:47:37	ID requested
⏵ 1	2020-03-20 00:56:48	announced

Jaswal S, Anand V, Kumar S, Bathla S, Dang AK, Kaushik JK, Mohanty AK, In-depth proteome analysis of more than 12,500 proteins in buffalo mammary epithelial cell line identifies protein signatures for active proliferation and lactation. Sci Rep, 10(1):4834(2020) [pubmed]

submitter keyword: Mammary gland;Buffalo

Subcellular;Proteomics

Secretome

Dr. Ashok Kumar Mohanty
contact affiliation	Proteomics and Structural Biology Lab, Animal biotechnology Center, National Dairy Research Institute, Karnal, Haryana, India
contact email	ashokmohanty1@gmail.com
lab head
Ashok Kumar Mohanty
contact affiliation	ICAR
contact email	ashokmohanty1@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013707
     1. Label: PRIDE project
     2. Name: Deep proteome analysis of more than 12000 proteins in buffalo mammary epithelial cells identifies protein signatures for active proliferation and lactation