PXD013702

PXD013702 is an original dataset announced via ProteomeXchange.

Title	Protein identification in 1000 U2OS cells
Description	In this study, we aim to optimize sample processing methods using a conventional in-solution proteomic sample processing workflow. We then compared protein idetnfication numbers withour improved sample processing methods versus the original method, in 1000 U2OS human osteosarcoma cells.
HostingRepository	PRIDE
AnnounceDate	2019-05-06
AnnouncementXML	Submission_2019-07-26_02:40:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu Lu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-03 03:44:54	ID requested
1	2019-05-06 06:24:58	announced
⏵ 2	2019-07-26 02:40:17	announced	Updated publication reference for PubMed record(s): 31318197.

Wu R, Xing S, Badv M, Didar TF, Lu Y, Step-Wise Assessment and Optimization of Sample Handling Recovery Yield for Nanoproteomic Analysis of 1000 Mammalian Cells. Anal Chem, 91(16):10395-10400(2019) [pubmed]

submitter keyword: 1000 cells, LC-MS, U2OS, osteosarcoma, sample recovery yield, sam ple processing

Yu Lu
contact affiliation	Department of Biochemistry and Biomedical Sciences McMaster University
contact email	yu.lu@mcmaster.ca
lab head
Yu Lu
contact affiliation	McMaster University
contact email	yu.lu@mcmaster.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD013702

PRIDE project URI

• PRIDE
  1. PXD013702
     1. Label: PRIDE project
     2. Name: Protein identification in 1000 U2OS cells