PXD013678

PXD013678 is an original dataset announced via ProteomeXchange.

Title	Haemaphysalis longicornis salivary gland LC-MSMS
Description	Ticks are notorious carriers of pathogens; these blood-sucking arthropods can spread a variety of deadly diseases. The salivary gland is the main organ in ticks, and this organ begins to develop rapidly when Ixodidae ticks suck blood. When these ticks reach a critical weight, the salivary glands stop developing and begin to degenerate. Specific developmental features of the salivary glands are regulated by multiple factors, such as hormones, proteins and other small molecular substances. In this study, we used iTRAQ quantitative proteomics to study dynamic changes in salivary gland proteins in female Haemaphysalis longicornis at four feeding stages: unfed, partially fed, semi-engorged, and engorged. Through bioinformatics analysis of a large number of proteins, we found that molecular motor- and TCA cycle-related proteins play an important role during the development of the salivary glands. The results of RNAi experiments showed that when dynein, kinesin, isocitrate dehydrogenase, and citrate synthase were knocked down, ticks were unable to suck blood normally. The structure and function of the salivary glands were also significantly affected. In addition, four proteins from H. longicornis were found to have very low homology with those from mammals, including humans. Therefore, it is expected that drugs or antibodies targeting these unique sequences can be designed to kill ticks.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-27_02:41:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaoshuang wang
SpeciesList	scientific name: Haemaphysalis longicornis; NCBI TaxID: 44386;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-30 08:07:59	ID requested
⏵ 1	2020-05-27 02:41:38	announced

Ren S, Zhang B, Xue X, Wang X, Zhao H, Zhang X, Wang M, Xiao Q, Wang H, Liu J, Salivary gland proteome analysis of developing adult female Haemaphysalis longicornis ticks: molecular motor and TCA cycle-related proteins play an important role throughout development. Parasit Vectors, 12(1):613(2019) [pubmed]

submitter keyword: Haemaphysalis longicornis,salivary gland,LC-MSMS

Hui Wang
contact affiliation	Hebei normal university,China
contact email	whui1981@163.com
lab head
xiaoshuang wang
contact affiliation	hebei normal university
contact email	wxsss9494@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD013678

PRIDE project URI

• PRIDE
  1. PXD013678
     1. Label: PRIDE project
     2. Name: Haemaphysalis longicornis salivary gland LC-MSMS