PXD013676

PXD013676 is an original dataset announced via ProteomeXchange.

Title	An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells
Description	The structural diversity of glycans on cells – the glycome – is vast and complex to decipher. Glycan arrays have played a pivotal role in exploring the informational content of glycans. Current glycan arrays display oligosaccharides generated by chemical and chemoenzymatic synthesis or isolated from biological sources and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources, produced through considerable efforts, and they display individual saccharides without the natural context of other glycans and glycoconjugates at the cell surface. We usedmaps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic and structural basis for glycan binding epitopes in a natural context. This expandable and self-renewable cell-based glycan array reports glycosyltransferase genes required (or blocking) for interactions through logic sequential biosynthetic steps, which not only predicts essential structural features of involved glycans including the glycoconjugate context, but importantly provides instructions for enzymatic synthesis, recombinant production, and genetic strategies to dissect biological functions. The broad utility of this cell-based glycan array and its comprehensive read-out is demonstrated by dissecting glycan-binding specificities of microbial adhesins, and the discovery power is demonstrated by uncovering higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.
HostingRepository	PRIDE
AnnounceDate	2019-06-24
AnnouncementXML	Submission_2019-06-27_02:01:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergey Vakhrushev
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-30 08:05:20	ID requested
1	2019-06-24 06:56:24	announced
⏵ 2	2019-06-27 02:01:52	announced	Updated publication reference for PubMed record(s): 31227230.

Narimatsu Y, Joshi HJ, Nason R, Van Coillie J, Karlsson R, Sun L, Ye Z, Chen YH, Schjoldager KT, Steentoft C, Furukawa S, Bensing BA, Sullam PM, Thompson AJ, Paulson JC, B, ü, ll C, Adema GJ, Mandel U, Hansen L, Bennett EP, Varki A, Vakhrushev SY, Yang Z, Clausen H, An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells. Mol Cell, 75(2):394-407.e5(2019) [pubmed]

submitter keyword: cell array, OrbiTrap, LC-MS, glycoprotein

Henrik Clausen
contact affiliation	Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, University of Copenhagen
contact email	hclau@sund.ku.dk
lab head
Sergey Vakhrushev
contact affiliation	Department of Cellular and Molecular Medicine
contact email	seva@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD013676

PRIDE project URI

• PRIDE
  1. PXD013676
     1. Label: PRIDE project
     2. Name: An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells