PXD013668

PXD013668 is an original dataset announced via ProteomeXchange.

Title	In vivo identification of GTPase interactors by mitochondrial relocalizationand proximity biotinylation
Description	The ~160 small GTPases that comprise the Ras superfamily include many major regulators of growth, membrane traffic and the cytoskeleton, and a wide range of diseases are caused by mutations in particular members. They function as switchable landmarks with the active GTP-bound form recruiting to the membrane a specific set of effector proteins. The location and level of the GTP-bound form is precisely controlled by upstream regulators that promote acquisition of GTP (GEFs) or its hydrolysis to GDP (GAPs). We report here MitoID, a method for identifying effectors and regulators by performing in vivo proximity biotinylation with mitochondrial-localized forms of the GTPases. Applying this to 11 human Rab GTPases identified many known effectors and GAPs, as well as putative novel interactors, with examples of the latter validated for Rab2, Rab5 and Rab9. We also show that MitoID can efficiently identify effectors and GAPs of members of other GTPase families such as Cdc42, RhoA, Rac1, Rheb, RalB and N-Ras, and can also identify GEFs by use of GDP-bound forms.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:54:33.006.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Skehel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-30 03:13:33	ID requested
1	2019-07-17 01:18:28	announced
⏵ 2	2024-10-22 04:54:37	announced	2024-10-22: Updated project metadata.

Gillingham AK, Bertram J, Begum F, Munro S, In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation. Elife, 8():(2019) [pubmed]

10.7554/elife.45916;

submitter keyword: Human, mitochondria GTPase, BirA*, Ras, lc-ms/ms, membranes, GEFs

Sean Munro
contact affiliation	Cell Biology, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK.
contact email	sean@mrc-lmb.cam.ac.uk
lab head
Mark Skehel
contact affiliation	MRC LMB
contact email	mskehel@mrc-lmb.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD013668

PRIDE project URI

• PRIDE
  1. PXD013668
     1. Label: PRIDE project
     2. Name: In vivo identification of GTPase interactors by mitochondrial relocalizationand proximity biotinylation