PXD013667

PXD013667 is an original dataset announced via ProteomeXchange.

Title	Dynamic response in the larval geoduck clam proteome to elevated pCO2
Description	Pacific geoduck (Panopea generosa) clams are found along the Northeast Pacific coast where they are significant components of coastal and estuarine ecosystems and the basis of a highly profitable aquaculture industry. The Pacific coastline, however, is also the sight of rapidly changing ocean habitat, including significant reductions in pH. To better understand the physiological impact of ocean acidification on geoduck clams, we characterized for the first time the proteomic profile of this bivalve during early larval development and compared it to that of larvae exposed to low pH.Geoduck larvae wer reared at pH 7.5 (ambient) or 7.1 in a commercial shellfish hatchery from day 6 to 19 post-fertilization , and sampled at six time points for an in-depth proteomics analysis using high-resolution data dependent analysis. We found that larvae reared at low pH were smaller than those reared at ambient pH, especially in the prodissoconch II phase of development, and displayed a delay in their competency for settlement. Proteomic profiles revealed that metabolic, cell cycle, and protein turnover pathways differed between the two pH, suggesting that differing phenotypic outcomes between pH 7.5 and 7.1 are likely due to environmental disruptions to the timing of molecular physiological events. In summary, ocean acidification likely caused an energetic stress on geoduck larvae, casuing a shift in physiological prioritization.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:13.091.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emma Timmins-Schiffman
SpeciesList	scientific name: Panopea generosa; NCBI TaxID: 1049056;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-30 03:12:58	ID requested
1	2019-05-04 03:09:03	announced
2	2020-01-20 00:43:38	announced	2020-01-20: Updated publication reference for DOI(s): 10.1002/ece3.5885.
⏵ 3	2023-11-14 08:50:13	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: pH,geoduck, DDA

Steven Roberts
contact affiliation	University of Washington, School of Aquatic and Fishery Sciences
contact email	sr320@uw.edu
lab head
Emma Timmins-Schiffman
contact affiliation	University of Washington
contact email	emsie25@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD013667

PRIDE project URI

• PRIDE
  1. PXD013667
     1. Label: PRIDE project
     2. Name: Dynamic response in the larval geoduck clam proteome to elevated pCO2