PXD013664

PXD013664 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide lysine succinylome profiling and comprehensive analysis of protein lysine co-modification in developing rice seeds
Description	Lysine succinylation has been recognized as a post-translational modification (PTM) in recent years. It is plausible that succinylation may have a vaster functional impact than acetylation due to bulkier structural changes and greater charge differences on the modified lysine residue. Currently, however, the quantity of identified succinylated proteins and their corresponding functions in cereal plants remain largely unknown. In this study, 854 lysine succinylation sites on 347 proteins have been identified by a thorough investigation in developing rice seeds. Six motifs were revealed as preferred amino-acid sequence arrangements for succinylation sites, and a noteworthy motif preference was discovered in proteins associated with different biological processes, molecular functions, pathways, and domains. Remarkably, heavy succinylation was detected on major seed storage proteins, in conjunction with key enzymes involved in central carbon metabolism and starch biosynthetic pathways for rice seed development. Conserved succinylated proteins were identified amongst varying organisms. Rice proteins with co-modifications of succinylation, acetylation, malonylation, crotonylation, and 2-hydroxyisobutyrylation were identified through a comprehensive comparison analysis. A striking number of highly conserved succinyl-proteins and multi-modified proteins were shown to be involved in vital metabolic events. Our study delivers a platform for expansive investigation of molecular networks administrating cereal seed development via PTMs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:55:49.673.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaonan fu
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	N6-succinyl-L-lysine
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-30 02:37:24	ID requested
1	2019-09-09 07:14:59	announced
⏵ 2	2024-10-22 04:55:51	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra119.001426;

Meng X, Mujahid H, Zhang Y, Peng X, Redo, ñ, a ED, Wang C, Peng Z, Comprehensive Analysis of the Lysine Succinylome and Protein Co-modifications in Developing Rice Seeds. Mol Cell Proteomics, 18(12):2359-2372(2019) [pubmed]

submitter keyword: Post-translational modification

Lysine succinylation

Succinylome

Rice

Storage nutrient

Seeds

Zhaohua Peng
contact affiliation	Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville MS, 39762, USA
contact email	zp7@BCH.msstate.edu
lab head
xiaonan fu
contact affiliation	zhejiang scientific technology universitiy
contact email	2016460649@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD013664

PRIDE project URI

• PRIDE
  1. PXD013664
     1. Label: PRIDE project
     2. Name: Proteome-wide lysine succinylome profiling and comprehensive analysis of protein lysine co-modification in developing rice seeds