⮝ Full datasets listing

PXD013637

PXD013637 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes
DescriptionGenes encoding Arabidopsis subunit homologs of TFIIH kinase module were labelled with coding sequences of GFP and mCherry (green and red fluorescent proteins) using fast-track recombineering. To illustrate the applicability of recombineering for accelerating the isolation and identification of plant protein complexes, proteins associated with CDKD;2-GFP, CYCLIN-H-mCherry and DNA replication-dependent HISTONE H3.1-mCherry complexes were purified on GFP-Trap and RFP-TRAP and analysed by LC-MS/MS mass spectrometry. The results confirmed association of know TFIIH subunit homologs with CDKD;2 and CYCLIN H, and identified subunits of CAF1 (CHROMATIN ASSEMBLY FACTOR 1) and ASF1A/B histone chaperon in complex with HISTONE H3.1.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:55:23.803.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSara Christina Stolze
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-25 07:46:11ID requested
12019-08-01 04:42:37announced
22024-10-22 04:55:24announced2024-10-22: Updated project metadata.
Publication List
10.1111/tpj.14450;
Hu Z, Ghosh A, Stolze SC, Horv, á, th M, Bai B, Schaefer S, Z, ü, ndorf S, Liu S, Harzen A, Hajheidari M, Sarnowski TJ, Nakagami H, Koncz Z, Koncz C, Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes. Plant J, 100(2):411-429(2019) [pubmed]
Keyword List
submitter keyword: Arabidopsis thaliana,fast-track recombineering, IP-MS
Contact List
Hirofumi Nakagami
contact affiliationBasic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact emailnakagami@mpipz.mpg.de
lab head
Sara Christina Stolze
contact affiliationPlant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne
contact emailstolze@mpipz.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013637
PRIDE project URI
Repository Record List
[ + ]