PXD013637 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes |
Description | Genes encoding Arabidopsis subunit homologs of TFIIH kinase module were labelled with coding sequences of GFP and mCherry (green and red fluorescent proteins) using fast-track recombineering. To illustrate the applicability of recombineering for accelerating the isolation and identification of plant protein complexes, proteins associated with CDKD;2-GFP, CYCLIN-H-mCherry and DNA replication-dependent HISTONE H3.1-mCherry complexes were purified on GFP-Trap and RFP-TRAP and analysed by LC-MS/MS mass spectrometry. The results confirmed association of know TFIIH subunit homologs with CDKD;2 and CYCLIN H, and identified subunits of CAF1 (CHROMATIN ASSEMBLY FACTOR 1) and ASF1A/B histone chaperon in complex with HISTONE H3.1. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:55:23.803.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sara Christina Stolze |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-25 07:46:11 | ID requested | |
1 | 2019-08-01 04:42:37 | announced | |
⏵ 2 | 2024-10-22 04:55:24 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1111/tpj.14450; |
Hu Z, Ghosh A, Stolze SC, Horv, á, th M, Bai B, Schaefer S, Z, ü, ndorf S, Liu S, Harzen A, Hajheidari M, Sarnowski TJ, Nakagami H, Koncz Z, Koncz C, Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes. Plant J, 100(2):411-429(2019) [pubmed] |
Keyword List
submitter keyword: Arabidopsis thaliana,fast-track recombineering, IP-MS |
Contact List
Hirofumi Nakagami |
contact affiliation | Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany |
contact email | nakagami@mpipz.mpg.de |
lab head | |
Sara Christina Stolze |
contact affiliation | Plant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne |
contact email | stolze@mpipz.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013637 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013637
- Label: PRIDE project
- Name: Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes