⮝ Full datasets listing

PXD013634

PXD013634 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe effects of Sindbis viral vectors on neuronal function
DescriptionViral vectors are attractive tools to express genes in neurons. Transduction of neurons with a recombinant, replication-deficient Sindbis viral vector is a method of choice for studying the effects of short-term protein overexpression on neuronal function. However, to which extent Sindbis by itself may affect neurons is not fully understood. We assessed effects of neuronal transduction with a Sindbis viral vector on the transcriptome and proteome in organotypic hippocampal slice cultures, and analyzed the electrophysiological properties of individual CA1 neurons, at 24h and 72h after viral vector injection. Whereas Sindbis caused substantial gene expression alterations, changes at the protein level were less pronounced. Alterations in transcriptome and proteome were predominantly limited to proteins involved in mediating anti-viral innate immune responses. Sindbis transduction did not affect the electrophysiological properties of individual neurons: the membrane potential, excitability and synaptic currents were similar between transduced and nontransduced CA1 neurons up to 72h after Sindbis injection. We conclude that Sindbis viral vectors are suitable for studying short-term effects of a protein of interest on electrophysiological properties of neurons, but not for studies on the regulation of gene expression.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:54:39.882.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSophie van der Spek
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-25 06:09:06ID requested
12019-08-26 00:31:53announced
22024-10-22 04:54:40announced2024-10-22: Updated project metadata.
Publication List
10.3389/fncel.2019.00362;
Uyaniker S, van der Spek SJF, Reinders NR, Xiong H, Li KW, Bossers K, Smit AB, Verhaagen J, Kessels HW, The Effects of Sindbis Viral Vectors on Neuronal Function. Front Cell Neurosci, 13():362(2019) [pubmed]
Keyword List
submitter keyword: Triple TOF, proteomics, hippocampus, mouse,sindbis virus
Contact List
Ka wan Li
contact affiliationThe department of Molecular and cellular neurobiology, Neuroproteomics, CNCR, Vrije University Amsterdam
contact emailk.w.li@vu.nl
lab head
Sophie van der Spek
contact affiliationCNCR, VU amsterdam
contact emails.j.f.vander.spek@vu.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013634
PRIDE project URI
Repository Record List
[ + ]