PXD013634

PXD013634 is an original dataset announced via ProteomeXchange.

Title	The effects of Sindbis viral vectors on neuronal function
Description	Viral vectors are attractive tools to express genes in neurons. Transduction of neurons with a recombinant, replication-deficient Sindbis viral vector is a method of choice for studying the effects of short-term protein overexpression on neuronal function. However, to which extent Sindbis by itself may affect neurons is not fully understood. We assessed effects of neuronal transduction with a Sindbis viral vector on the transcriptome and proteome in organotypic hippocampal slice cultures, and analyzed the electrophysiological properties of individual CA1 neurons, at 24h and 72h after viral vector injection. Whereas Sindbis caused substantial gene expression alterations, changes at the protein level were less pronounced. Alterations in transcriptome and proteome were predominantly limited to proteins involved in mediating anti-viral innate immune responses. Sindbis transduction did not affect the electrophysiological properties of individual neurons: the membrane potential, excitability and synaptic currents were similar between transduced and nontransduced CA1 neurons up to 72h after Sindbis injection. We conclude that Sindbis viral vectors are suitable for studying short-term effects of a protein of interest on electrophysiological properties of neurons, but not for studies on the regulation of gene expression.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:54:39.882.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophie van der Spek
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-25 06:09:06	ID requested
1	2019-08-26 00:31:53	announced
⏵ 2	2024-10-22 04:54:40	announced	2024-10-22: Updated project metadata.

10.3389/fncel.2019.00362;

Uyaniker S, van der Spek SJF, Reinders NR, Xiong H, Li KW, Bossers K, Smit AB, Verhaagen J, Kessels HW, The Effects of Sindbis Viral Vectors on Neuronal Function. Front Cell Neurosci, 13():362(2019) [pubmed]

submitter keyword: Triple TOF, proteomics, hippocampus, mouse,sindbis virus

Ka wan Li
contact affiliation	The department of Molecular and cellular neurobiology, Neuroproteomics, CNCR, Vrije University Amsterdam
contact email	k.w.li@vu.nl
lab head
Sophie van der Spek
contact affiliation	CNCR, VU amsterdam
contact email	s.j.f.vander.spek@vu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013634

PRIDE project URI

• PRIDE
  1. PXD013634
     1. Label: PRIDE project
     2. Name: The effects of Sindbis viral vectors on neuronal function