PXD013631

PXD013631 is an original dataset announced via ProteomeXchange.

Title	Temporary bioadhesion in the proseriate flatworm Minona ileanae
Description	Flatworms can very rapidly attach to and detach from many substrates. In the presented work, we analyzed the adhesive system of the marine proseriate flatworm Minona ileanae. We used light-, scanning-, and transmission electron microscopy to analyze the morphology of the adhesive organs, which are located at the ventral side of the tail plate. We performed transcriptome sequencing and differential RNA-seq for the identification of tail-specific transcripts. Using in situ hybridization expression screening, we identified nine transcripts that were expressed in the cells of the adhesive organs. Knock-down of five of these transcripts by RNA interference led to a reduction of the animal's attachment capacity. Adhesive proteins in footprints were confirmed using mass spectrometry and antibody staining. Additionally, lectin labelling of footprints revealed the presence of several sugar moieties. Furthermore, we determined a genome size of about 560 megabases for M. ileanae. We demonstrated the potential of Oxford Nanopore sequencing of genomic DNA as a cost-effective tool to identify the number of repeats within an adhesive protein and to combine transcripts that were fragments of larger genes. A better understanding of the molecules involved in flatworm bioadhesion paves the way towards developing innovative glues with reversible adhesive properties.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-11_17:19:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Ladurner
SpeciesList	scientific name: Minona ileanae; NCBI TaxID: 60013;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-25 02:20:09	ID requested
⏵ 1	2019-11-11 17:19:20	announced

Pjeta R, Wunderer J, Bertemes P, Hofer T, Salvenmoser W, Lengerer B, Coassin S, Erhart G, Beisel C, Sobral D, Kremser L, Lindner H, Curini-Galletti M, Stelzer CP, Hess MW, Ladurner P, Temporary adhesion of the proseriate flatworm Minona ileanae. Philos Trans R Soc Lond B Biol Sci, 374(1784):20190194(2019) [pubmed]

curator keyword: Biological

submitter keyword: Bioadhesion, Platyhelminthes, Flatworms

Peter Ladurner
contact affiliation	Institute of Zoology and Center of Molecular Bioscience Innsbruck, University of Innsbruck, 6020 Innsbruck, Austria
contact email	peter.ladurner@uibk.ac.at
lab head
Peter Ladurner
contact affiliation	University of Innsbruck
contact email	peter.ladurner@uibk.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD013631

PRIDE project URI

• PRIDE
  1. PXD013631
     1. Label: PRIDE project
     2. Name: Temporary bioadhesion in the proseriate flatworm Minona ileanae