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PXD013627

PXD013627 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDe novo Classification of Mouse B Cell Types using Surfaceome Proteotype Maps
DescriptionSystem-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when sample is limiting. We miniaturized and automated the previously described Cell Surface Capture technology increasing sensitivity, reproducibility, and throughput. We used this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and used targeted flow cytometry to uncover developmental cell subpopulations.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:48:07.756.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaik Müller
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-24 23:46:14ID requested
12019-12-11 05:36:04announced
22020-09-28 00:11:25announced2020-09-28: Updated publication reference for PubMed record(s): 31844046.
32024-10-22 04:48:08announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41467-019-13418-5;
van Oostrum M, M, ü, ller M, Klein F, Bruderer R, Zhang H, Pedrioli PGA, Reiter L, Tsapogas P, Rolink A, Wollscheid B, Classification of mouse B cell types using surfaceome proteotype maps. Nat Commun, 10(1):5734(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Flow Cytometry, cell surface capture,cell surface proteome, HRM, B-cell development, DDA, mouse model, classification, affinity purification, DIA, LC-MS, proteotype, autoCSC, CSC, surfaceome
Contact List
Bernd Wollscheid
contact affiliationInstitute of Molecular Systems Biology & Department of Health Sciences and Technology, ETH Zurich, Switzerland
contact emailbernd.wollscheid@hest.ethz.ch
lab head
Maik Müller
contact affiliationETH Zurich
contact emailmaimuell@ethz.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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