PXD013627 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | De novo Classification of Mouse B Cell Types using Surfaceome Proteotype Maps |
Description | System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when sample is limiting. We miniaturized and automated the previously described Cell Surface Capture technology increasing sensitivity, reproducibility, and throughput. We used this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and used targeted flow cytometry to uncover developmental cell subpopulations. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:48:07.756.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maik Müller |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-24 23:46:14 | ID requested | |
1 | 2019-12-11 05:36:04 | announced | |
2 | 2020-09-28 00:11:25 | announced | 2020-09-28: Updated publication reference for PubMed record(s): 31844046. |
⏵ 3 | 2024-10-22 04:48:08 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41467-019-13418-5; |
van Oostrum M, M, ü, ller M, Klein F, Bruderer R, Zhang H, Pedrioli PGA, Reiter L, Tsapogas P, Rolink A, Wollscheid B, Classification of mouse B cell types using surfaceome proteotype maps. Nat Commun, 10(1):5734(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Flow Cytometry, cell surface capture,cell surface proteome, HRM, B-cell development, DDA, mouse model, classification, affinity purification, DIA, LC-MS, proteotype, autoCSC, CSC, surfaceome |
Contact List
Bernd Wollscheid |
contact affiliation | Institute of Molecular Systems Biology & Department of Health Sciences and Technology, ETH Zurich, Switzerland |
contact email | bernd.wollscheid@hest.ethz.ch |
lab head | |
Maik Müller |
contact affiliation | ETH Zurich |
contact email | maimuell@ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD013627 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013627
- Label: PRIDE project
- Name: De novo Classification of Mouse B Cell Types using Surfaceome Proteotype Maps