PXD013627

PXD013627 is an original dataset announced via ProteomeXchange.

Title	De novo Classification of Mouse B Cell Types using Surfaceome Proteotype Maps
Description	System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when sample is limiting. We miniaturized and automated the previously described Cell Surface Capture technology increasing sensitivity, reproducibility, and throughput. We used this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and used targeted flow cytometry to uncover developmental cell subpopulations.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:48:07.756.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maik Müller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-24 23:46:14	ID requested
1	2019-12-11 05:36:04	announced
2	2020-09-28 00:11:25	announced	2020-09-28: Updated publication reference for PubMed record(s): 31844046.
⏵ 3	2024-10-22 04:48:08	announced	2024-10-22: Updated project metadata.

10.1038/s41467-019-13418-5;

van Oostrum M, M, ü, ller M, Klein F, Bruderer R, Zhang H, Pedrioli PGA, Reiter L, Tsapogas P, Rolink A, Wollscheid B, Classification of mouse B cell types using surfaceome proteotype maps. Nat Commun, 10(1):5734(2019) [pubmed]

curator keyword: Biological

submitter keyword: Flow Cytometry, cell surface capture,cell surface proteome, HRM, B-cell development, DDA, mouse model, classification, affinity purification, DIA, LC-MS, proteotype, autoCSC, CSC, surfaceome

Bernd Wollscheid
contact affiliation	Institute of Molecular Systems Biology & Department of Health Sciences and Technology, ETH Zurich, Switzerland
contact email	bernd.wollscheid@hest.ethz.ch
lab head
Maik Müller
contact affiliation	ETH Zurich
contact email	maimuell@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD013627

PRIDE project URI

• PRIDE
  1. PXD013627
     1. Label: PRIDE project
     2. Name: De novo Classification of Mouse B Cell Types using Surfaceome Proteotype Maps