PXD013620

PXD013620 is an original dataset announced via ProteomeXchange.

Title	The dynamics of Atm signalling dictate cellular fate decisions after DNA damage
Description	DNA damage activates diverse cellular responses – either protective or deleterious –that ultimately promote or inhibit proliferation. How the distinct responses conferring crucial cell fate decisions are chosen is unclear. Using a systems approach, we demonstrate that the dynamic features of Atm dependent DNA double-strand break (DSB) signalling response dictate cellular outcome. Combining temporal phosphoproteome and nascent transcriptome analyses after low or high DNA-damage-load, we discovered that some responses, such as Tp53 activation, have an activation threshold and others arise independently of DNA-damage-load. Using DSB repair deficient cells, we show that persistent DSBs alter the kinetics – but not the amplitude – of Atm signalling. Thus, we demonstrate that pathway choices are dictated by the signalling dynamics and hence cell fate decisions are responsive to DNA-damage-load and repair capacity of the cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:30:08.612.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bharath Sampadi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	L-methionine sulfoxide; 6x(13)C; 6x(13)C; phosphorylated residue; (4,4,5,5-(2)H4)-L-lysine; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; 6x(13)C labeled L-arginine; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-24 06:01:53	ID requested
⏵ 1	2024-10-07 13:30:09	announced

10.3390/CELLS11233794;

submitter keyword: Phosphoproteomics, Mdc1, post-translational modification, mass spectrometry, Mouse, ionizing radiation,Biological, Phosphorylation, LC-MSMS, mESCs, Atm, SILAC, signal transduction, Chek2

Harry Vrieling
contact affiliation	DNA damage responses and cancer lab Department of Human Genetics Leiden University Medical Center Eithovenweg 20 2333ZC Leiden The Netherlands
contact email	h.vrieling@lumc.nl
lab head
Bharath Sampadi
contact affiliation	Leiden University Medical Center
contact email	b.k.sampadi@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD013620

PRIDE project URI

• PRIDE
  1. PXD013620
     1. Label: PRIDE project
     2. Name: The dynamics of Atm signalling dictate cellular fate decisions after DNA damage