PXD013618

PXD013618 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Activity-based protein profiling of xylan-degrading glycoside hydrolases in Aspergillus secretomes
Description	Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides towards breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling (ABPP) provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of b-xylosidases and endo-xylanases in the secretome of Aspergillus niger, by the use of chemical probes inspired by the b-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.
HostingRepository	MassIVE
AnnounceDate	2019-09-27
AnnouncementXML	Submission_2021-02-02_15:49:53.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Adam Dowle
SpeciesList	scientific name: Aspergillus niger; NCBI TaxID: 5061;
ModificationList	Oxidation; Carbamidomethyl; unknown modification
Instrument	maXis

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-24 04:09:30	ID requested
⏵ 1	2021-02-02 15:49:53	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Activity-based probe, Glycosidase, Biofuel, Industrial enzyme, Xylanase

submitter keyword: Activity-based probe, Glycosidase, Biofuel, Industrial enzyme, Xylanase

Contact List

Gideon J. Davies
contact affiliation	University of York
contact email	gideon.davies@york.ac.uk
lab head
Adam Dowle
contact affiliation	University of York
contact email	adam.dowle@york.ac.uk
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083714/